六六六对红壤和紫色土中细菌群落多样性及结构的影响
作者:
作者单位:

1.南京农业大学土壤有机污染物控制与修复研究所;2.山东省沂南县第三中学

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基金项目:

国家自然科学基金项目(41771523,41977121)资助


Effects of Hexachlorocyclohexane on Bacterial Community in Red and Purple Soils in Terms of Variaty and Structure
Author:
Affiliation:

1.Institute of Organic Contaminant Control and Soil Remediation, Nanjing Agricultural University;2.The Third Middle School of Yinan

Fund Project:

Supported by the National Natural Science Foundation of China (Nos. 41771523 and 41977121)

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    摘要:

    尽管六六六已被禁用多年,但由于难降解、易持留的特性,其在土壤中检出率仍较高。残存的六六六对土壤细菌群落多样性和组成结构的影响一直备受关注,相关认识仍待深化。采用小麦盆栽试验,借助高通量测序技术,模拟研究了不同施药浓度(0、600、1 500 g?hm-2)下六六六对旱地红壤和紫色土中细菌群落多样性和结构的影响。Alpha指数分析结果表明,在培养初期,施用六六六显著提高红壤细菌群落多样性,而对紫色土无显著影响;但随培养时间延长,在第42天时细菌群落多样性均恢复至对照组(CK)水平。细菌群落组成分析显示,红壤中优势菌门为变形菌门(Proteobacteria)和放线菌门(Actinobacteria)。分析OTUs(可执行分类操作单元)丰度发现,六六六提高了红壤放线菌门和酸杆菌门(Acidobacteria)的多样性和丰度,从而导致红壤细菌群落多样性指数上升;紫色土中优势菌门为变形菌门和酸杆菌门。六六六增加了红壤中固氮菌Bradyrhizbium(慢生根瘤菌)丰度,600和1 500 g?hm-2六六六处理组较对照分别增长了150%和180%;紫色土中,随六六六处理浓度增大,固氮菌相对丰度降低。六六六引起土壤细菌群落和结构的变化,对红壤细菌群落的影响要强于紫色土。研究结果为明确六六六污染土壤的微生态效应提供理论依据。

    Abstract:

    【Objective】 Although hexachlorocyclohexane (HCH) has been banned as pesticide in agriculture for decades, its residue, still high in concentration, has been frequently observed in soil due to its property of persistentcet, So it remains to be an important task to well elucidate how HCH affects soil microbial communities in diversity and structure, and a topic of great significance to ecological health of the agro-environment systems.【Method】In this work, a greenhouse experiment was conducted, in red and purple soils grown with wheat, spiked with HCH at a rate of 0, 600, or 1500 g/ha. Soil bacterial community structure and diversity in the soils were determined using the high throughput sequencing technique. Total DNA in the soil samples were extracted using the Power Soil DNA Isolation Kit, a product of from MOBIO and tested with 1% agarose gel electrophoresis for quality. Compositions of the soil microbial communities were characterized with the sequencing technique.【Result】Alpha index analysis revealed that HCH significantly increased diversity of the bacterial community in the red soil, while no significant effect was observed in the purple soil at the beginning of the wheat cultivation. However, after 42 days of cultivation, no significant differences were found between the soils spiked with HCH and CK in soil bacterial community diversity in the soils. Analysis of bacterial community compositions showed that Proteobacteria and Actinobacteria were the dominant phyla at the phyluma level, and HCH increased the diversity of Actinobacteria and Acidobacteria in the red soil, while Proteobacteria and Acidobacteria were the dominant ones in the purple soil. Relative abundance of Bradyrhizbium in red the soil increased with the spiking rate of HCH, and its abundance did about 150% and 180% in the soils spiked with 600 and 1 500 g?hm-2 HCH, as compared ing with that in the control treatment. However, in the purple soil, relative abundance of the nitrogen-fixing bacteria decreased with the addition of HCH.【Conclusion】The residue of HCH obviously induced the changes in diversity and structure of the of soil bacterial community in the soils, and the impact was more obvious in the red soil than in the purple soil. The findings provide a basis for understanding of the micro-ecological effects and safety assessment of HCH in the soil environments.

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唐凯迪,高乃征,刘娟,凌婉婷.六六六对红壤和紫色土中细菌群落多样性及结构的影响[J].土壤学报,DOI:10.11766/trxb202008200416,[待发表]
Tang Kai Di, Gao Nai Zheng, Liu Juan, Ling Wan Ting. Effects of Hexachlorocyclohexane on Bacterial Community in Red and Purple Soils in Terms of Variaty and Structure[J]. Acta Pedologica Sinica, DOI:10.11766/trxb202008200416,[In Press]

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  • 收稿日期:2020-07-24
  • 最后修改日期:2020-10-11
  • 录用日期:2020-11-25
  • 在线发布日期: 2020-12-10
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