TRXB土壤学报Acta Pedologica Sinica0564-3929土壤学报编辑部中国南京trxb-60-4-114610.11766/trxb202112080664S154.38A研究论文Research Articles放牧对黄土高原典型草原冬季牧场甲烷氧化菌类群及活性的影响Grazing Effect on Activity and Diversity of Soil Methanotrophs in Winter Pastures of the Loess Plateau王玉芳WANGYufang
Soil methanotrophs are the only biological sink of atmospheric methane (CH4). Grazing can regulate the methane oxidation activity of the soil by affecting the abundance and diversity of soil methanotrophs.
Method
In this study, we collected soil samples in winter pasture from different intensities of continuous grazing on typical grasslands in the Loess Plateau and used laboratory incubation and high-throughput sequencing to determine methane oxidation activity, composition and abundance of soil methanotrophs.
Result
The results showed that the grassland was CH4 sink, and the medium grazing (MG) and high grazing (HG) intensity increased the rate of methane oxidation. Meanwhile, the mean abundance of methanotrophs in HG and MG also increased significantly compared with CK. The results of high-throughput sequencing showed that grazing had a significant impact on the diversity of methanotrophs, Upland soil cluster gamma (USCγ) was the dominant methanotrophic group under different grazing intensities, and a small amount of Methylocaldum and Methylocystis were also present. Pearson correlation analysis showed that methane oxidation rate (MOR) showed a significant positive correlation with soil moisture content and \begin{document}$ {\text{NO}}_3^ - $\end{document}-N content (P < 0.05), and significantly positively correlated with the absolute amount of USCγ (P < 0.01), which indicated that USCγ played a major role in the process of methane uptake in this grassland.
Conclusion
This study proved that grazing can improve the methane sink function of typical grasslands in the Loess Plateau.
黄土高原草地甲烷氧化放牧USCγThe Loess PlateauGrasslandMethane oxidationGrazingUSCγ国家自然科学基金项目91751204国家自然科学基金项目41877062国家自然科学基金项目41530857国家自然科学基金项目(91751204,41877062,41530857)资助the National Natural Science Foundation of China91751204the National Natural Science Foundation of China41877062the National Natural Science Foundation of China41530857Supported by the National Natural Science Foundation of China(Nos. 91751204, 41877062, and 41530857)
Soil CH4 oxidation potential estimated by atmospheric concentration CH4 incubated with soils from different grazing intensities(a), the atmospheric CH4 oxidation rate(b)and soil methanotroph abundance estimated by pmoA gene abundance in the soil at different grazing intensities(c)
Non-metric multidimensional scaling(NMDS)plot illustrating the β diversity of methanotrophs(a), Correlation analysis between CH4 oxidation potential and the absolute number of USCγ by pmoA gene(b)and Pearson correlation illustrating the relationships between the absolute abundance of methanotrophs, CH4 oxidation potential and soil properties of 16S rRNA(c)genes
P < 0.05;**表示P < 0.01。]]>
P < 0.05;** means P < 0.01.
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