%0 Journal Article %T 耕作措施对陇中旱农区土壤细菌群落的影响 %T Effects of Tillage on Soil Bacterial Community in the Dryland Farming Area of Central Gansu %A 邓超超 %A 李玲玲 %A 谢军红 %A 彭正凯 %A 王进斌 %A 颉健辉 %A 沈吉成 %A EUNICE Essel %A DENG,Chaohcao %A LI,Lingling %A XIE,Junhong %A pengzhengkai %A WANG,Jinbin %A XIE,Jianhui %A SHEN,Jicheng %A EUNICE,Essel %J 土壤学报 %J ACTA PEDOLOGICA SINICA %@ 0564-3929 %V 56 %N 1 %D 2019 %P 207-216 %K 耕作措施;秸秆还田;长期定位试验;土壤细菌群落 %K Tillage practice; Straw retuning; Long-term experiment ; Soil bacterial community %X 为探究耕作措施对陇中旱农区麦豆轮作系统土壤细菌多样性的影响,借助2001年在陇中旱农区建立的不同耕作措施长期定位试验,应用高通量测序技术比较传统耕作(T)与3种保护性耕作(免耕(NT)、传统耕作+秸秆还田(TS)和免耕+秸秆覆盖(NTS))对麦豆轮作体系土壤有机碳、全氮、微生物生物量碳、微生物生物量氮及细菌群落结构影响。结果表明:1)相对于传统耕作,秸秆还田显著增加0~10 cm、10~30 cm土壤有机碳、全氮、微生物量碳和微生物量氮含量,其中NTS处理0~10 cm、10~30 cm土层有机碳、全氮、微生物量碳和微生物量氮含量较传统耕作分别提高28.27%、114.16%、13.51%、49.14%和39.86%、98.05%、10.78%、40.72%。2)酸杆菌门(26.42%)、变形菌门(19.86%)和放线菌门 (19.44%)为陇中旱农区麦豆轮作系统土壤细菌的主要优势种群,NTS处理下酸杆菌门、放线菌门和变形菌门丰度较传统耕作显著提高35.11%、33.77%和30.17%。 3)与传统耕作相比,保护性耕作提高了0~30 cm土层土壤细菌的Observed species指数、Chao指数、香农指数、辛普森指数,以NTS处理提高最为显著。因此,在陇中旱农区推广以免耕秸秆覆盖为主的保护性耕作措施有利于增加土壤碳氮固存、提高土壤细菌群落丰度和多样性和土壤的生物活性,促进农业的可持续发展。 %X 【Objective】The objective of the study was to investigate effects of tillage on soil bacterial diversity and soil properties in upland under a wheat-bean rotation system.【Method】A long-term stationary field experiment was started on tillage in an upland in Central Gansu in 2001. The experiment was designed to have four treatments, i.e. Treatment T (conventional tillage), Treatment NT (no tillage), Treatment TS (conventional tillage with straw returned), and Treatment NTS (no tillage with straw mulched).【Result】Results show: 1) Compared with Treatment T, Treatments NTS and TS significantly increased the contents of soil organic carbon, soil total nitrogen, soil microbial biomass carbon and soil microbial biomass nitrogen in the 0~10 cm and 10~30 cm soil layers, especially Treatment NTS, which increased the contents of soil organic carbon, soil total nitrogen, soil microbial biomass carbon and soil microbial biomass nitrogen by 28.27%, 114.16%, 13.51% and 49.14%, respectively, in the 0~10 cm soil layer and by 39.86%, 98.05%, 10.78% and 40.72%, respectively, in the 10~30 cm soil layer; 2) Acidobacteria (26.42%), Proteobacteria (19.86%) and Actinomycetes (19.44%) were the main dominant groups of bacteria, accounting for 26.42%, 19.86% and 19.44%, respectively, of the total population of the soil bacterial community in the upland. Treatment NTS increased the abundance of the three groups by 35.11%, 33.77% and 30.17%, respectively; and 3) Compared with the traditional tillage, the three conservation tillage practices increased the soil microbial in the 0~30 cm soil layer in Observed-species index, Chao index, Shannon index and Simpson index, and the effect of treatment NTS was the most significant.【Conclusion】Therefore, conservation tillage practices such as no-tillage with straw mulching would not only increase soil carbon and nitrogen sequestration, improve abundance and diversity of the soil bacterial community and soil biological activity, but can also promote sustainable development of the agriculture in the dryland farming area of Central Gansu. %R 10.11766/trxb201802050073 %U http://pedologica.issas.ac.cn/trxb/home %1 JIS Version 3.0.0