%0 Journal Article %T 铜和强力霉素复合污染对土壤微生物与酶活性的影响 %T Impacts of Combined Pollution of Copper and Doxycycline on Activities of Soil Microbes and Enzymes %A 陈欣瑶,肖祖飞,祝妍华,武剑,华倩雯,张园 %A CHEN,Xinyao %A XIAO,Zufei %A ZHU,Yanhua %A WU,Jian %A HUA,Qianwen %A ZHANG,Yuan %J 土壤学报 %J ACTA PEDOLOGICA SINICA %@ 0564-3929 %V 58 %N 4 %D 2021 %P 957-967 %K 强力霉素;铜;土壤微生物呼吸;酶活性;抗生素抗性基因 %K Doxycycline;Copper;Soil microbial respiration;Enzyme activity;Antibiotic resistance genes %X 当前土壤环境中重金属和抗生素的广泛共存及二者复合存在所诱导出的细菌抗性,与单一物质的污染相比,均能够加剧对土壤质量和作物安全的破坏。在在实验室模拟培养条件下,向土壤中加入不同浓度的重金属(铜)和抗生素(强力霉素),探讨抗生素和重金属复合污染对土壤微生物呼吸、脲酶、蔗糖酶和过氧化氢酶活性和四环素抗性基因的丰度等土壤微生物指标的影响。结果显示,在整个培养期(30 d)内,铜和强力霉素单一及复合污染均会显著抑制土壤微生物呼吸强度,对脲酶活性主要表现为促进作用,对蔗糖酶、过氧化氢酶活性主要为抑制作用,对过氧化氢酶活性的抑制强度明显大于蔗糖酶。综合而言,铜和强力霉素的复合污染相对于单一污染对上述微生物指标的影响较大,强力霉素的加入可以促进铜对微生物呼吸或酶活性的初始影响。此外,该研究还表明添加为400 mg·kg-1铜可以提高强力霉素在土壤培养中后期诱导的抗性基因相对丰度的能力水平。本研究从微生物角度定量探讨铜与强力霉素单一及复合污染对土壤微生物指标的影响程度,以期为重金属与抗生素协同污染的土壤构建微生物预警体系,并为土壤修复和风险评估工作提供理论依据。 %X [Objective] Nowadays, more attention should be paid to the situation of agricultural environment and the quality and safety issues of agricultural produces because more and more various pollutants are entering into the agricultural soil. Among the pollutants, heavy metals and antibiotics are two of the most important types of pollutants in the farmland soil. In this study, soil microorganisms are used as indicators to illustrate impacts of combined pollution of heavy metals and antibiotics on soil environment.[Method] An in-lab analogue incubation experiment was conducted in this study, with the soil spiked with doxycycline (DOX, 0, 8 and 15 mg·kg-1) and copper (Cu, 0, 100 and 400 mg·kg-1), singly or combinedly, at a varying rate as the representative pollutants of antibiotic and heavy metal, respectively. Effects of the pollutants on respiration of soil microbes, activities of soil enzymes, like urease, sucrase and catalase, and abundance of tet ARGs (tetracycline-resistance genes) were investigated.[Result] Results show that throughout the whole incubation period (30 days), the pollution of DOX and Cu, either singly or combinedly, inhibited significantly respiration of soil microbes in intensity, and activities of sucrase and catalase, but stimulated that of urease, and the effect was apparently catalase than on sucrase. To sum up, the effects of combined pollutions is much higher than that of singe pollutions and the addition of DOX stimulated the initial effect of Cu on respiration of soil microbes or activities of soil enzymes. Correlation analysis shows that the activity of catalase/urease was significantly and negatively related to Cu concentration and urease activity significantly and negatively correlated with sucrase activity. Besides, it was also found that the total relative abundance of four types of antibiotic resistance genes (ARGs) decreased first and then turned backwards, which was probably owing to the tie lag of about 7-15 days the soil system needs to adapt and age after the addition of the pollutants. Compared with other treatments, the addition of high concentrations of Cu and DOX significantly increased the abundance of ARGs at the mid-and late-stages of the incubation, especially the relative abundance of tetA and tetW. The addition of a high concentration (400 mg·kg-1) of Cu improved the ability of DOX to induce high relative abundance of tet ARGs.[Conclusion] All the findings in this study indicate that combined pollution of Cu and DOX has dramatic impacts on functions of the soil microbial system. %R 10.11766/trxb202002200514 %U http://pedologica.issas.ac.cn/trxb/home %1 JIS Version 3.0.0