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引用本文:李敬王,陈林,马东豪,张丛志,张佳宝.潮土长期不同施氮水平对秸秆降解及其细菌群落结构的影响[J].土壤学报,DOI:10.11766/trxb202103150145,[已录用].
李敬王,陈林,马东豪,张丛志,张佳宝.Influences of long-term different N application rates on straw decomposition and bacterial community structure in a fluvo-aquic soil[J].Acta Pedologica Sinica,DOI:10.11766/trxb202103150145,[In Press]
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潮土长期不同施氮水平对秸秆降解及其细菌群落结构的影响
李敬王, 陈林, 马东豪, 张丛志, 张佳宝
中国科学院南京土壤研究所
摘要:
农田过量施氮所引发的问题已经引起广泛关注,但长期施氮后土壤无机氮水平状况及其对秸秆降解的作用尚不清楚。本研究以中国科学院封丘农业生态实验站长期(2005~2018年)施氮肥(5个施氮水平:0(N0)、150(N1)、190(N2)、,230(N3)和270 (N4)kg ha-1 yr-1)的潮土为研究对象,开展短期(50天)的秸秆降解-土壤培育实验。培育期间监测秸秆碳的矿化、土壤无机氮(硝态氮和铵态氮)、可溶性有机碳、微生物生物量碳的动态变化,利用高通量测序测定细菌群落结构。结果表明,长期施氮后,土壤无机氮含量和秸秆碳的矿化率随施氮水平的升高而增加。不同长期施氮水平的土壤细菌群落结构呈现显著差异。网络分析揭示:秸秆降解过程中细菌群落内部物种间的共现模式随长期施氮水平发生改变,具体体现为长期高施氮水平下细菌群落彼此间的负相关得到了加强;同时,变形菌主导地位减弱、酸杆菌主导地位增强。综上,土壤无机氮含量、细菌群落结构及物种之间的关系随着长期施氮水平的不同发生了改变。本研究探究了长期不同施氮水平下土壤中无机氮的水平状况、秸秆降解状况以及秸秆降解过程中土壤细菌的生物特性,以期为秸秆还田和科学施氮提供一定的数据支撑和思路启示。
关键词:  长期施氮  土壤无机氮残留  秸秆降解  细菌  细菌群落
DOI:10.11766/trxb202103150145
分类号:
基金项目:国家自然科学基金项目(41807017)、中国科学院战略性先导科技专项子课题(XDA24020104)
Influences of long-term different N application rates on straw decomposition and bacterial community structure in a fluvo-aquic soil
lijingwang, chenlin, madonghao, zhangcongzhi, zhangjiabao
Institute of Soil Science, Chinese Academy of Sciences
Abstract:
【【Objective】The problems caused by excessive nitrogen (N) application in agriculture has been of growing concern, but the soil mineral N level and its effect on straw decomposition after long-term N application remain unclear. 【Method】We used a fluvo-aquic soil treated for 14 years (2005-2018) with N at the rates of 0 (N0), 150 (N1), 190 (N2), 230 (N3) and 270 (N4) kg N ha-1 yr-1, and conducted a 50-day laboratory incubation experiment with and without straw addition. Soil CO2 emission was determined during the incubation, soil mineral N (nitrate and ammonium nitrogen), dissolved organic carbon (DOC), microbial biomass carbon (MBC) were measure before and after the incubation, and the bacterial community structure was determined using amplicon sequencing of the 16S rRNA genes. 【Result】The results showed that soil mineral N content and straw decomposition efficiency increased with increasing N application rates and the bacterial community composition was significantly (P < 0.05) influenced by N application rates. The network analysis revealed that the co-occurrence pattern among species within the bacterial community during straw decomposition changed with the N application level, and the negative association among bacterial communities was strengthened under a high N application level. At the same time, the dominant position of Proteobacteria was weakened and that of Acidobacteria was enhanced. 【Conclusion】 The relationship among soil mineral N content, bacterial community structure and species changed with the difference of long-term N application level. The results of this study provide guidelines for the use of straws to amend soils under N application.
Key words:  Long-term nitrogen application  Soil mineral nitrogen residual  Straw decomposition  Bacteria  Bacterial community