TY - JOUR ID - 10.11766/trxb201503030643 TI - Microbial Biodiversity in Rhizosphere of Lycium bararum L. Relative to Cultivation History AU - NA Xiaofan AU - ZHENG Guoqi AU - PENG Li AU - LEI Chuanyi AU - YANG Hongyan AU - MA Yu AU - ZHAO Qiang and SHI Shuofan VL - 53 IS - 1 PB - SP - 241 EP - 252 PY - JF - ACTA PEDOLOGICA SINICA JA - UR - http://pedologica.issas.ac.cn/trxben/home?file_no=trxb201412110643&flag=1 KW - 宁夏枸杞;微生物多样性;根际土壤 KW - Lycium bararum L.; Microbial diversity; Rhizosphere soil AB - The rhizosphere is a critical interface where exchange of substance takes place between plants roots and their surrounding soil. In the rhizosphere, interactions between the plant and soil microbes, though affected by a series of factors, such as physic-chemical properties of the rhizospheric soil, genotype of their host plant, can be beneficial to growth of the plant, the microbes or both. However, it is still not very clear how long-term cultivation of Lycium bararum L. would affect soil microbial community structure in the rhizosphere of the plant. Therefore, rhizospheric soil samples were collected from Lycium bararum L. fields different in cultivation history (5 a, 10 a and 15 a) in a farm of Nanliang, Ningxia, China, for analysis of physic-chemical properties, such as pH, electrical conductivity, SOM, total salt, total and readily available N, P and K, etc. Results showed that pH remained unchanged in all the fields, while total salt content, total and readily available phosphorus and electrical conductivity in the soil increased significantly with the age of cultivation. Total genomic DNA was isolated from the rhizosphere soil using a Power Soil DNA Isolation Kit for sequence analysis of V4 sections of 16S rDNA as indicator of bacterial diversity and ITS2 sections of 18S rDNA gene as indicator of fungal diversity, with the aid of the Illumina MiSeq system. Results of the sequencing were assembled and clustered with the FLASH, QIIME, and UPARSE pipeline software packages. In the end, is was found that the α diversity of the rhizosphere bacteria community did not vary much between the fields different in cultivation history, but fungal diversity did, and decreased with the cultivation going on from 5 a to 10 a (p﹤0.05). For analysis of changes in microbial community structure at the phyla and genus levels, the software of RDA Classifier to denote each OUT by species. It was found that Proteobacteria (22.2%), Crenarchaeota (15.1%), Bacteroidetes (13.9%), Acidobacteria (12.4%), Chloroflexi (10.3%), Gemmatimonadetes (4.8%), Actinobacteria (4.7%), Planctomycetes (4.0%) and Verrucomicrobia (2.0%) were the dominate bacterial groups and Ascomycota (29.5%) and Basidiomycota (11.7%) were the dominant fungal group in the rhizosphere of the plant. However, about 55.8% of the fungi found in the rhizosphere were still unknown in the taxonomy. Besides, the sequencing further demonstrated that soil microbial community structure in the rhizosphere varied sharply between the fields different in cultivation history and stood out uniquely in each field from the others. The variation between fields different in cultivation history was particularly significant in terms of the ratio of Acidobacteria, Actinobacteria, Bacteroidetes, Fimicutes, Chloroflexi, Crenarchaeota, Cyanobacteria, Gemmatimonadetes, Proteobacteria, Ascomycota, Basidiomycota and Zygomycota in the rhizospheric microbial community (p﹤0.05). The protion of unknown fungal groupsin taxonomy was much higher in the 10 a (76.6%) and 15 a (61.4%) Lycium bararum L. fields than in the 5 a (32.5%) fields (p﹤0.05). The analysis at the genera level also shows that the ratios of 27 genera of bacteria and 16 genera of fungi changed with the cultivation going on (p﹤0.05). In order to further analyze relationships of soil community, with soil physic-chemical properties, hundred-grain weight and cultivation history, SPSS 16.0 software was used to work out pearson’s correlation coefficients between the data, which show that cultural history is significantly related to richness of Firmicutes, Proteobacteria, Verrucomicrobia, Basidiomycota and Zygomycota (p﹤0.05), content of total phosphorus in the rhizosphere soil is to richness of Firmicutes, Gemmatimonadetes, Proteobacteria, Basidiomycota, Zygomycota (p﹤0.05) and content of readily available phosphorus is to richness of Acidobacteria, Gemmatimonadetes, Ascomycot, Basidiomycota and Zygomycota (p﹤0.05). The findings further demonstrated that the three factors, cultural year, total phosphorus and readily available phosphorus, are the key factors affecting soil microbial composition in the rhizosphere of Lycium bararum L. To sum up, cultivation history affects soil microbial community structure more than soil microbial diversity in the rhizosphere soil of Lycium bararum L., which may be related to soil phosphorus metabolism in the rhizosphere. ER -