【Objective】Effects of organic manure on soil nutrients, crop yield, and composition and stability of soil aggregates in the soil under mono-cropping soybean were explored through a seven-year field experiment, designed to have three treatments, in respect to type of organic manure, i.e. JF (Composted chicken droppings), JG (corn stalk) and JZ (Fungi residues).【Method】 In this study, soil samples were collected and analyzed regularly for variations of nutrient contents, composition and stability of soil aggregates, and mean weight diameter of water stable soil aggregates. 【Result】 Results show that application of organic manure improved soil nutrient contents in the soil under mono-cropping of soybean, and altered composition of soil aggregates significantly by increasing the fraction (0.25～2 mm) of aggregates obviously. All the treatments were higher than CK in content of alkaliyzable N, readily available P, readily available potassium, organic matter, microbial biomass C, microbial biomass N and microbial biomass P. Treatment JF was the most obvious in such effect on content of alkalyzable nitrogen, or 13.85% higher than CK; Treatment JG was the most on content of readily available phosphorus or 4.13% higher than CK; Treatment JF was the most on content of readily available potassium or 8.97% higher than CK; Treatment JG was the most on content of organic matter or 7.28% higher than CK. Treatment JZ, though not so good as the other two, was still significantly higher than CK in nutrient content. Application of the organic manures improved yield of soybean, and Treatment JF was the most remarkable in this effect. The application also effectively raised the content of soil microbial biomass carbon in the soil and Treatment JZ was the most in this effect. Treatment JF and JG did have some effect, too,, but not so significant. All the three treatments increased significantly the content of nitrogen in the soil, and Treatment JF was more obvious than Treatment JG and JZ in this effect, which indicates that organic manures can significantly increase the content of soil microbial biomass nitrogen. Treatment JG was the most effective in improving the content of soil microbial biomass phosphorus in the soil and Treatments JF and JZ were, too, though not so significantly. Treatments JG and JZ significantly improved the metabolic entropy of soil microorganisms, but Treatment JF was lower than CK in metabolic entropy. Mean weight diameter of the soil aggregate in the three treatments increased, exhibiting an order of JF > JG > JZ. The three treatments increased the fraction (> 1 mm) of soil aggregates more than the others In Treatment JF, JG and JZ the fraction (> 1mm) of aggregates accounted for 29.52%, 38.97% and 29.07%, respectively, of the soil aggregate stability index, and the fraction (> 0.25 mm) of aggregates was significantly higher than that in CK in stability index, which suggests that the organic manures applied into the soil can effectively improve stability of the soil aggregates, and the proportion of water-stable soil aggregates. It can be seen that application of the organic manures is very effective in increasing mean weight diameter and water stability of the soil aggregates in soybean fields. The three types of organic manure all can effectively improve soil aggregate stability, and Treatment JG is the most effective, increasing by 89.79% or being 4.293 In terms of soil aggregate stability index, the treatments exhibite an order of JG (4.293) > JZ (3.788) > JF (3.720) > CK (2.262). Application of the organic manures significantly improve soil aggregation, increasing the fraction (> 0.25 mm) of water stable aggregates and its mean weight diameter, preservation probability and stability index.【Conclusion】The organic manures can significantly improve soil fertility, and composition and water stability of the soil aggregates in the soybean fields. This study is expected to be able to provide certain reference for improving soil fertility and increasing soybean yield, which is of great significance to overcoming the obstacle of mono-cropping of soybean.