【Objective】In recent years, biochar has extensively been used as an important fertilization amendment to improve crop quality and fertilizer use efficiency, but little is applied in the yellow soil for pod pepper cultivation in Guizhou. Therefore, this paper was oriented mainly to explore effects of application of biochar on nitrogen content in pod pepper growing in yellow soil of Guizhou.【Method】A field experiment was conducted to study effects of combined application of biochar and N fertilization on yield, quality, nutrient accumulation and nitrogen fertilizer utilization efficiency of pod pepper. The experiment was designed to have four treatments, different in combination ratio, i.e., CF₁₀₀B₀ (Chemical N fertilizer 100% and Biochar 0%), CF₉₀B₁₀ (Chemical N fertilizer 90% and Biochar 10%), CF₈₅B₁₅ (Chemical N fertilizer 85% and Biochar 15%) and CF₈₀B₂₀ (Chemical N fertilizer 80% and Biochar 20%).【Result】Results show that compared with Treatment CF₁₀₀B₀, Treatment CF₉₀B₁₀ increased the yield of pod pepper, by 7.3% in fresh weight and by 2.5% in dry weight while Treatments CF₈₅B₁₅ and CF₈₀B₂₀ decreased the yield slightly. Combined application significantly affected the contents of nitrate and Vc in the fruit of pod pepper. The content of nitrate in the fruit decreased by 4.8%~8.9% in Treatments CF₉₀B₁₀, CF₈₅B₁₅ and CF₈₀B₂₀, while the content of Vc increased by 9.6% only in Treatment CF₉₀B₁₀. But no difference was found between the treatments in content of reducing sugar and free amino acids. In addition, the treatments, especially CF₉₀B₁₀, increases PFP_N by 2.08~2.62 kg·kg^-1, but lowered AE_N and RE_N in a trend with rising biochar ratio, so Treatment CF₉₀B₁₀ was the highest in AE_N and RE_N, being 7.7 kg·kg^-1 and 40.3% respectively.【Conclusion】On the whole, the application of biochar to substitute portion of nitrogen fertilizer can effectively ensure stable yield and N utilization efficiency of pod pepper in Guizhou. The use of biochar to substitute 10% of the chemical nitrogen fertilizer is the best in biological effect and hence recommended as the most suitable formula of fertilization for pod pepper production in the yellow soil of Guizhou.