Community Composition and Diversity of Fungi in Soils under Different Types of Pinus koraiensis Forests
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National Natural Science Foundation of China(Nos. 31670627, 31770656) and the Fundamental Research Funds for the Heilongjiang Province Universities(No.2017-KYYWF-0597)

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    Abstract:

    【Objective】To investigate community composition and diversity of soil fungi in relation to type of Pinus koraiensis forest, a field survey was carried out in the Liangshui National Nature Reserve, where there are four types of Pinus koraiensis forest, that is, Picea koraiensis and Abies fabri-Pinus koraiensis forest, Tilia amurensis-Pinus koraiensis forest, Pinus koraiensis plantations and Pinus koraiensis secondary forest.【Method】Soil samples were collected under the four types of Pinus koraiensis forests for analysis by means of ITS (Internal Transcribed Spacer) sequence based Illumina miseq high-throughput sequencing.【Result】Results show that out of the 16 soil samples collected from the soils under the four types of Pinus koraiensis forests, a total of 505 299 high quality sequences and 534 359 effective sequences and a total of 1316 fungal OTUs were obtained. In the soils of the four types of forests, besides a huge number of unidentified fungi, Ascomycota and Basidiomycota were found to be in dominancy. Distribution of the latter varied from soil to soil (P<0.05).In the soils of the four types of forests, besides a huge number of unidentified fungi, Ascomycota and Basidiomycota were found to be in dominancy.There were significant differences in the distribution of Basidiomycota in the four forest types (P<0.05).And at the genus level, Leptodontidium, Simplicillium, Sebacina, Russula, Penicillium , and Cryptococcus were the dominant ones.The LDA scores of LEfSe analysis show that there were 19 fungi genera with significantly different abundance in four forest types, among 10 genera in the soil under Tilia amurensis-Pinus koraiensis forest and one genera in the soil under natural Pinus koraiensis secondary forest. Alpha diversity analysis shows that the soils under primitivePinus koraiensis forests (Picea koraiensis and Abies fabri-Pinus koraiensis forest and Tilia amurensis-Pinus koraiensis forest) were significantly higher than that under Pinus koraiensis secondary forest in fugal community richness index (Ace and Chao1 index). Beta diversity analysis shows that the soils differed significantly in fungi community composition (r=0.537, P=0.001), but no much difference was found between the two primitive Pinus koraiensis forests and between the Pinus koraiensis plantation and natural Pinus koraiensis secondary forest. Soil pH, moisture content, total porosity, total nitrogen and total organic carbon were the main factors influencing soil fungal community composition in the four forest soils (P<0.05).Correlation analysis of the top 20 dominant genus of fungi and soil physicochemical factors shows that 6 genera were significantly related to soil total nitrogen in relative abundance. Among the six genera, Russula, Suillus and unclassified-f-Thelephoraceae were positively related while Malassezia , Simplicillium and Penicillium were negatively.【Conclusion】There were significant differences in the composition of soil fungal community composition of four types of Pinus koraiensi forests, but there was no statistically difference in the composition of between the two primitive Pinus koraiensis forests and between the Pinus koraiensis plantation and natural Pinus koraiensis secondary forest. Soil total nitrogen may be the main factor affecting the composition of soil fungal communities of four forest types, followed by total organic carbon. All the findings in this study may serve as a theoretical basis for protection of primitive Pinus koraiensis forests, artificial rehabilitation of vegetation and exploitation of the fungal resources in the soil.

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CHEN Xiubo, ZHU Dequan, ZHAO Chenchen, ZHANG Lulu, CHEN Lixin, DUAN Wenbiao. Community Composition and Diversity of Fungi in Soils under Different Types of Pinus koraiensis Forests[J]. Acta Pedologica Sinica,2019,56(5):1221-1234.

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History
  • Received:October 29,2018
  • Revised:April 16,2019
  • Adopted:June 25,2019
  • Online: July 03,2019
  • Published: