引用本文:郑华,欧阳志云,方治国,赵同谦.BIOLOG在土壤微生物群落功能多样性研究中的应用[J].土壤学报,2004,41(3):456-461. DOI:10.11766/trxb200309010320
Zheng Hua,Ouyang Zhiyun,Fang Zhiguo,Zhao Tongqian.APPLICATION OF BIOLOG TO STUDY ON SOIL MICROBIAL COMMUNITY FUNCTIONAL DIVERSITY[J].Acta Pedologica Sinica,2004,41(3):456-461. DOI:10.11766/trxb200309010320
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BIOLOG在土壤微生物群落功能多样性研究中的应用
郑华, 欧阳志云, 方治国, 赵同谦
中国科学院生态环境研究中心系统生态重点实验室, 北京, 100085
摘要:
微生物功能多样性信息对于明确不同环境中微生物群落的作用具有重要意义,而微生物群落的定量描述一直是微生物学家面临的最艰巨的任务之一。目前,以群落水平碳源利用类型为基础的BIOLOG氧化还原技术为研究土壤微生物群落功能多样性提供了一种简单、快速的方法,并得以广泛应用。但它仍然是一种以培养为基础的方法,显示的代谢多样性类型也不一定反映整个土壤微生物群落的功能多样性。因此,这种方法优点明显,缺陷也存在,并且在应用过程中还有很多关键的操作要点与技巧。本文综述了BIOLOG研究土壤微生物群落功能多样性的原理、BIOLOG研究土壤微生物群落功能多样性的方法与技巧、应用过程中容易产生的问题及可能克服的办法,同时还提出了值得进一步研究的问题。旨在促进对BIOLOG测定土壤微生物群落功能多样性的了解,为正确运用这种方法开展土壤微生物群落功能多样性研究提供科学依据和理论指导。
关键词:  BIOLOG  土壤微生物群落  功能多样性
基金项目:* 中国科学院知识创新工程重要方向项目(KZCX3-SW-424);国家重点基础研究发展规划项目(G2000046807);国家自然科学基金项目(30230090)资助
APPLICATION OF BIOLOG TO STUDY ON SOIL MICROBIAL COMMUNITY FUNCTIONAL DIVERSITY
Zheng Hua, Ouyang Zhiyun, Fang Zhiguo, Zhao Tongqian
Key Laboratory of Systems Ecology, Research Center for Eco-Environmental Sciences, Beijing 100085, China
Abstract:
In order to understand the role of microbial communities in different environments it is essential to have knowledge of functions and functional diversity of microbial communities.However, the quant itative description of microbial communities is one of the most difficult tasks facing microbial ecologists.Now the BIOLOG redox technique proposes a simple and quick method for studying soil microbial community function diversity, based on the community-level carbon source utilization patterns.But BIOLOG is also a culture-based method, and the presentation of metabolic diversity patterns doesn't necessarily reflect the functional diversity of the soil microbial communities.Consequently, there are many problems as well as benefits using the approach, and a series of skills are needed for the application.The application of BIOLOG to the study on soil microbial community functional diversity is summarized in this paper, including theories, procedures, problems, and countermeasures during the application.The problems worthy of further research are also put forward.The objective of the study is to accelerate understanding how to apply BIOLOG properly to the study on soil microbial community functional diversity and provide a scientific foundation and theoretical guide for further research of the subject correctly.
Key words:  BIOLOG  Soil microbial community  Functional diversity