The soil agrochemical properties and microbiota of three degraded grasslands (Kobresia pygmaea meadow,Dasiphora fruticosa shrub and Festuca sinensis grassland) were studied.The main results are summarized as flliows:(1) There existed obvious vertical distribution of the contents of N,P,K,organic matters and pH value as well as microorganism numbers in the soils.Soil agrochemical properties were significantly correlated with soil microorganism numbers.(2) Soil fertility and the numbers of soil microorganisms,decreased with grassland degradation.(3) N and P were deficient but K and organic matter were rich in soils of the three degraded grasslands.And (4) The soil microbiota varied obviously with grassland degradation.Weakly degraded grassland had more soil microorganism genera than the strongly degraded one,and the microbiota were similar in the grasslands with the similar degradation degrees.