The interactions between Pseudomonas Jluorescens X16L2 and Arbuscular Mycorrhizal Fungi (AMF) Glomus mosseae in the rhizosphere of wheat planted in the rhizobox and plastic box are studied by techniques of luminescent enzyme gene marker system, detecting of luminescence intensity and Succinate Dehydrogenase byeing. Because there is an inhibitory effect of Glomus mosseae on population density and physiological activity of Pf.X16L2, the mycorrhizosphere effect on Pf.X16L2 are lowered.Pf.X16L2 can promote the mycorrhizal infective ratio of Gl.mosseae on wheat roots but drops the Succinate Dehydrogenase activity of AMF mycelia. They can promote the growth of wheat during the middle and later growth stages.