The high efficiency degrading bacteria Pseudomonas and Bacilus were obtained by circulation culture.The conditions that influence degradative efficiency were studied.98.7% of pacloutrazol can be degraded naturally in sterile medium under vibration condition 99.8% of pacloutrazol can be degraded by degrading bacteria.Pacloutrazol can be degraded down to CO₂ by mixed degrading bacteria.If plenty of oxygen and light are adsorbed by soil,after 35d about 86.2% of pacloutrazol can be degraded and biomass can reach 89% of general level in soil.