Studies were carried out on influences of replacement of 25% NO3--N with NH4+-N in nutrient solution (enhanced ammonium nutrition) on tomato growth and activity of such key enzymes in N assimilation and organic acids as nitrate reductase (NR), glutamine synthetase(GS) and phosphoenolpyruvate carboxylase (PEPcase) in tomato leaves and fruits sampled at different developmental stages. The results showed that the replacement of 25% NO3--N with NH4+-N led to a significant increase in fruit fresh weight, NH4+-N content in both leaves and fruits and soluble protein content in fruits, while led to decrease signifi cantly in NO3--N content in tomato leaves. Meanwhile, significant enhancement of the activity of PEPcase in both leaves and fruits and of GS in leaves were observed but not much change in GS activity in tomato fruits was. NR activity was of ten observed to decrease dramatically in both fruit and leaves in enhanced ammonium treatment.
董园园,董彩霞,卢颖林,缪辰,沈其荣. NH4+-N部分代替NO3--N对番茄生育中后期氮代谢相关酶活性的影响[J].土壤学报,2006,43(2):261-266. DOI:10.11766/trxb200501250214 Dong Yuanyuan, Dong Caixia, Lu Yinglin, Miao Chen, Shen Qirong. INFLUENCE OF PARTIAL REPLACEMENT OF NO3--N WITH NH4+-N IN NUTRIENT SOLUTION ON ENZYME ACTIVITY IN NITROGEN ASSIMILATION OF TOMATO AT DIFFERENT GROWING STAGES[J]. Acta Pedologica Sinica,2006,43(2):261-266.复制