To overcome limitations of the traditional enrichment culturing method for degrading bacteria isolation, two strains of bacteria, AG1 and ADG1, capable of degrading atrazine efficiently, were isolated by diluting and plating atrazine-contaminated soils directly onto plates coated with soil extracts and atrazine.They grew in medium with atrazine as the sole sources of nitrogen, carbon and energy, degrading 1 000 mgL^-1 of atrazine within 44 h and 48 h, respectively, and could also grew with some other s-triazine herbicides as sole nitrogen sources.Based on their 16S rDNA sequences analysis and their physiologic and biochemical characteristics, AG1 and ADG1 were identified as Arthrobacter spp..Results of amplifying their degrading genes with PCR, showed that atrazine metabolic genotype of AG1 and ADG1 were both combination of trzN and atzBC.It is the first report in the country about atrazine-degrading bacteria with such metabolic genotypes.