类黄酮对AM真菌侵染菌丝生长及酶活性的影响
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* 国家自然科学基金项目(30270051);湖北省教育厅重点研究项目(2004D001)资助


IMPACT OF HESPERITIN ON INFECTION HYPHAL GROWTH AND ENZYME ACTIVITY OF AM FUNGUS
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    摘要:

    类黄酮(hesperitin)能显著促进AM真菌[Glomus intraradices(G.i)、Acaulospora laevis (A.l)G.i+A.l]对宿主植物(玉米、棉花)根段的侵染,提高菌丝琥珀酸脱氢酶(Succinate dehydrogenase,SDH)和碱性磷酸酶(Alkaline phosphatase,ALP)的活性。当用15nmolL-11、50nmolL-1、1.5μmolL-1的类黄酮处理时,第6周取样,G.i对玉米根段的侵染率分别为76.2%、84.5%、75.8%(对照为45.9%),菌丝SDH酶活性分别为68.4%、75.9%、67.4%(对照为40.7%),菌丝ALP酶活性分别为45.8%、51.4%、45.1%(对照为27.1%);G.i对棉花根段的侵染率分别为85.2%8、8.9%8、3.8%(对照为59.8%),菌丝SDH酶活性分别为76.8%、81.2%、75.0%(对照为53.1%),菌丝ALP酶活性分别为51.2%、53.7%4、9.4%(对照为35.2%)。同时,类黄酮能显著增加土壤中AM真菌菌丝的总量,对宿主植物(玉米、棉花)的生物量也有一定的影响。

    Abstract:

    In a pot experiment, it was found that host infection rates, and activities of hyphal SDH (succinate dehydrogenase) and ALP (alkaline phosphatase) enzymes of Glomus intraradices (G.i), Acaulospora laevis (A.l) and G.i + A.l were significantly enhanced by hesperitin.After treatment with 15 nmol L-1, 150 nmol L-1, and 1.5μ mol L-1 hesperitin, respectively, samples of maize and cotton roots were collected for test at the 6th week.Results of the tests showed that with the maize roots, the G.i infection rates were 76.2%, 84.5% and 75.8% (the control was 45.9%), hyphal SDH activity of G.i were 68.4%, 75.9% and 67.4% (the control was 40.7%), and hyphal ALP activity of G.i were 45.8%, 51.4% and 45.1% (the control was 27.1%), respectively.And with the cotton roots, the G.i infection rates were 85.2%, 88.9% and 83.8% (the control was 59.8%), hyphal SDH activity were 76.8%, 81.2% and 75.0% (the control was 53.1%), hyphal ALP activity were 51.2%, 53.7% and 49.4% (the control was 35.2%), respectively.And hesperitin could increase significantly total amount of hyphae of AM fungus in the soil.The biomass of maize and cotton could also be affected by hesperitin.

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董昌金,姚发兴,赵斌.类黄酮对AM真菌侵染菌丝生长及酶活性的影响[J].土壤学报,2006,43(3):473-477. DOI:10.11766/trxb200501250317 Dong Changjin, Yao Faxing, Zhao Bin. IMPACT OF HESPERITIN ON INFECTION HYPHAL GROWTH AND ENZYME ACTIVITY OF AM FUNGUS[J]. Acta Pedologica Sinica,2006,43(3):473-477.

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  • 收稿日期:2005-01-25
  • 最后修改日期:2005-05-10
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  • 在线发布日期: 2013-02-25
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