* 国家自然科学基金“30500010”项目;江苏省自然科学基金“BE2003343,BE2002303”项目资助
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从生产甲磺隆的农药厂内采取污泥,经驯化富集后筛选到一株能高效降解甲磺隆的细菌FLDA,根据表型特征、生理生化特性及16SrDNA序列同源性分析,将FLDA初步鉴定为假单胞菌(Pseudomonas.sp)。该菌能在含甲磺隆(30mgL-1)的基础盐液体培养基中降解甲磺隆,5d降解率达72.6%,该菌降解甲磺隆的最适pH为7.0,最适温度为30℃,该菌降解甲磺隆的速率和起始接种量呈正相关。酶的定域实验表明,该菌中甲磺隆水解酶为胞内酶。FLDA投加土壤,可提高土壤中甲磺隆的降解速率。
A strain of FLDA capable of degrading metsulfuron-methyl aerobically was isolated from sludge collected from a pesticide (metsulfuron-methyl) plant.Based on analysis of its 16S rDNA,morphology,physiological and biochemical characteristics,strain FLDA was identified preliminarily as Pseudomonas sp.This strain of bactera could degrade 72.6% of 30mgL-1 metsulfuron-methyl in liquid medium within 5days.The optimal pH and temperature for metsulfuron-methyl degradation was 7.0 and 30℃,respectively.The degradation rate was related positively to initial inoculation rate.Enzyme distribution experiment showed that the metsulfuron 2 methyl degradeing enzyme in the bacterium was endoenzyme.Strain FLDA could be used to degrade metsulfuron-methyl in soil.
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黄星,何健,潘继杰,孙纪全,顾立锋,李顺鹏.甲磺隆降解菌FLDA的分离鉴定及其降解特性研究[J].土壤学报,2006,43(5):821-827. DOI:10.11766/trxb200506280517 Huang Xing, He Jian, Pan Jijie, Sun Jiquan, Gu Lifeng, Li Shunpeng. ISOLATION AND IDENTIFICATION OF METSULFURON-METHYL DEGRADING STRAIN FLDA AND ITS DEGRADATING CHARACTERISTICS[J]. Acta Pedologica Sinica,2006,43(5):821-827.
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- 收稿日期:2005-06-28
- 最后修改日期:2005-09-25
- 在线发布日期: 2013-02-25
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