高寒草地不同扰动生境土壤微生物氮素生理群数量特征研究
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* 甘肃省科学技术攻关项目(2GS035-A41-001-04);国家自然科学基金项目(30371021)资助


POPULATIONS OF SOIL NITROGEN BACTERIA GROUPS IN ALPINE STEPPE OF DIFFERENT DISTURBED HABITATS IN TIANZHU
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    摘要:

    采用平板表面涂抹法和稀释法对天祝高寒草地5种不同干扰生境(围栏内、围栏外、多年生草地、一年生草地和鼠丘地)土壤微生物氮素生理群(氨化细菌、硝化细菌、自生固氮菌和反硝化细菌)数量分布特征进行了测定,结果表明:1)同一扰动生境,不同空间层次草地土壤微生物各氮素生理群数量变化较大,0~20cm数量大于20~40cm,一般前者为后者的1.44~7.32倍;且不同氮素生理群之间数量差异很大,氨化细菌>自生固氮菌>反硝化细菌>硝化细菌;2)不同扰动生境,相同空间层次草地土壤微生物各氮素生理群数量差异也较大,变幅在1.27~9.29倍;且不同扰动生境对各氮素生理群的影响不同,对硝化细菌数量影响最大,反硝化细菌和氨化细菌次之,自生固氮菌较小;3)不同生境土壤微生物氮素生理群数量以一年生草地总数量最大,多年生和围栏内草地次之,鼠丘地和围栏外草地最少;4)不同扰动生境,不同空间层次,虽然不同生理群微生物数量各有特点,但总趋势为:0~20cm,7月份数量较小,8月份上升至最大,9月份下降,20~40cm数量变化趋势与0~20cm相似,但变幅显著变小。

    Abstract:

    Populations and distribution of soil nitrogen bacteria groups(ammonifying bacteria,nitrobacteria,free nitrogen fixing bacteria and denitrobacteria)in alpine steppe of different disturbed habitats in Tianzhu were investigated and analyzed with the plate count and MPN methods.Results show 1)Populations of soil nitrogen bacteria groups varied greatly with soil depth. They were much greater(1.44 to 7.32 times)in 0~20 cm soil layer than in 20~40 cm one;and they also varied sharply from group to group,forming a decreasing order of ammonifying bacteria>free azotobacteria>denitrobacteria>nitrobacteria; 2)They differed sharply from habitat to habitat in the range of 1.27~9.29 times,and moreover the effect of habitat on population of nitrobacteria was much stronger than on those of denitrobacteria and ammonifying bacteria;3)The total population of the nitrogen bacteria groups was the highest in the annual artificial grassland,which was followed by perennial grassland,fenced grassland,rodent hill and un-fenced grassland in a decreasing order;and 4)Regardless of difference in habitat,spatial distribution and groups,the populations of soil nitrogen bacteria groups showed a general saddle-shaped tend,i.e.within the 0~20 cm soil layer,they were low in J uly,rose up the peak in August and declined in September,and within the 20~40 cm soil layer, they show the same but smaller in amplitude.

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姚拓,龙瑞军,师尚礼,张德罡.高寒草地不同扰动生境土壤微生物氮素生理群数量特征研究[J].土壤学报,2007,44(1):122-129. DOI:10.11766/trxb200511300118 Yao Tuo, Long Ruijun, Shi Shangli, Zhang Degang. POPULATIONS OF SOIL NITROGEN BACTERIA GROUPS IN ALPINE STEPPE OF DIFFERENT DISTURBED HABITATS IN TIANZHU[J]. Acta Pedologica Sinica,2007,44(1):122-129.

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  • 收稿日期:2005-11-30
  • 最后修改日期:2006-02-18
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  • 在线发布日期: 2013-02-25
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