With GPS and GIS method,the distribution of soil nitrogen at microscale,mesoscale and macroscale was studied in Lianshui Basin,Xingguo County,Jiangxi Province.The results suggest that both the total nitrogen and available nitrogen showed normal or lognormal distribution at the three different scales,but their means increased along the scale up,In the multi-scales,mean total nitrogen contents were 0.60 g kg^-1,0.73 g kg^-1 and 0.83 g kg^-1 respectively,and mean available nitrogen were 64.8 mg kg^-1,66.3 mg kg^-1 and 80.2 mg kg^-1,respectively.The spatial variability of soil total nitrogen and available nitrogen showed obvious scale effect with the different semivariogram parameters and spatial distribution for the multiscales.The combined sampling method of griding with stratifying is suitable for the study of spatial variability of soil nitrogen at the multi 2 scales in hilly area.Their reasonable sample points were 82,6%,71.2% and 58.1% of the initial sampling points under the 90% confidence level,and those were 95.7%,94.2% and 89.2% under the 95% confidence level,separately.