A Fusarium oxysporum-selective cultivation medium was used to investigate numbers of Fusarium oxysporum in soils under polytunnel greenhouse cultivation different in vegetable species,different in cultivation history,different in culture systems and different in depth.Results show that the number in the soil under cucumber was as high as 1.6×10⁴cfug^-1 dry soil,significantly higher than that in the soils under eggplant and under celery.And the number tended to increase with the years of cultivation,and was 22% higher in the soil 4 years in history than in the soil only 1 year. It was much lower in the aggregate than in the soil,being only 1.1×10×10⁴cfug^-1 dry soil. Maybe greenhouse vegetable cultivation using aggregate instead of soil is one way for the country to develop greenhouse vegetables in the future. The number of Fusarium oxysporum decreased with the increase in soil depth,indicating that Fusarium oxysporum rarely moved into deeper soil. All these findings may serve as primary microbiological bases for sustainable utilization of greenhouse vegetable soils in the future.