不同退化程度高寒草原土壤微生物活性变化特征研究
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国家自然科学基金项目(40461005、40761015);国家科技支撑计划重大项目(2007BAC06B04);教育部重点研究项目(206140)资助


VARIATION OF SOIL MICROBIAL ACTIVITIES IN ALPINE STEPPES DIFFERENT IN DEGRADATION INTENSITY IN THE NORTH TIBET PLATEAU
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    摘要:

    以紫花针茅草原为研究对象,选择成土母质、土壤质地一致的区域4个,分别在冷季、暖季于每个区域的正常草地和轻度、中度、严重退化草地内按网格法采集土壤样品,分析测定了土壤微生物(细菌、真菌、放线菌)数量、土壤微生物量(C、N)、土壤酶(纤维素酶、脲酶、碱性磷酸酶)活性.结果表明,高寒、干旱条件下,草地退化程度、季节变化对高寒草原土壤生物活性均具有显著影响.暖季、冷季土壤细菌数量、微生物量(C、N)、酶活性(纤维素分解酶、脲酶、碱性磷酸酶)间均存在着相似甚至高度相关的变化趋势,暖季土壤生物活性基本呈轻度退化草地>正常草地>中度退化草地>严重退化草地;暖季真菌、放线菌数量与微生物量、酶活性间分呈不同程度的正相关和负相关,冷季则呈相反趋势.不同季节间,冷季土壤生物活性较暖季总体呈显著下降趋势.其中,正常草地和轻度、中度、严重退化草地土壤细菌暖季/冷季比分别为206.0、251.7、18.4和87.4,真菌、放线菌分别为14.7、1132、0.6、0.9和0.1、10.5、10.0、14.9.微生物量(C、N)暖季/冷季比均呈轻度退化草地>正常草地>中度退化草地>严重退化草地,但微生物量氮的季节差异较大;暖季、冷季BC/BN比值亦基本呈同一趋势,冷季各类草地BC/BN值均明显高于暖季;各类草地BC/TC、BN/TN除严重退化草地外均呈暖季>冷季的趋势.暖季土壤脲酶活性远高于其他酶类,且暖季/冷季比(31.5~781.5)差异极大;冷季土壤纤维素分解酶,特别是碱性磷酸酶活性普遍高于暖季,暖季/冷季比分别在0.46~1.01和0.40~1.37之间.

    Abstract:

    Dynamics of soil microorganism s in stipa pu rpurea alp ine steppes different in degradation intensity in North Tibet Plateau were studied.Four p lots were selected,coincident in parent rock and soil texture,but different in degradation intensity,i.e.non-degraded(ND),slightly degraded(SD),moderately degraded(MD) and severely degraded(VD).Soil samp les were taken using the grid method from the four p lots in both warm and cold seasons.Results indicate that under alp ine arid conditions,both degradation intensity and seasonal variation have significant influences on soil microbial activities in alp ine steppe.A similar trend of variation of soil bacteria population,microbial biomass(carbon(C) and nitrogen(N)) and soil enzyme(cellulase,urease and alkaline phosphatase) activities as a whole was found in both warm and cold seasons,and so was high correlation between the two.In the warm season,the steppes were in the order of SD > ND >MD > VD in soil microbial activity.The numbers of fungi and actinomycetes were positively correlated with soil microbial biomass,but negatively with soil enzyme activity to a varying extent in warm season,while an opposite trend was found in cold season.The soil microbial activities in cold season declined significantly,compared with those in warm season.The ratio of soil bacteria in warm season to that in cold season in the four grades of steppes was 206 10,251.7,18.4 and 87.4,respectively,of fungi,14.7,1.132,0.6 and 0.9 and of actinomycetes,0.1,10.5,10.0 and 14.9.With respect to ratio of BC(microbial biomass C) and BN(microbial biomass N) in warm-season to those in cold-season,SD was the highest and followed by ND,MD,and then VD.and sharp variation of BN was observed between sea-sons.The ratios of BC/BN in cold season and in warm season were more or less the same in variation,but the BC/BN.ratio was much higher in cold season than in warm season.However,the values of BC/TC and BN/TN were higher in cold season than in warm season for all of the four grasslands,except VD,which showed a reverse trend.Soil urease was much higher than other soil enzymes in activity in warm season.The ratio of soil urea activity in warm season to that in cold season ranged from 31.5 to 781.5.The activity of soil cellulolytic enzyme,especially alkaline phosphatase,were higher in cold season than in warm season,and the ratio of the activity in warm season to that in cold season ranged from 0.46 to 1.01 for soil cellulolytic enzyme and 0.40 to 1.37 for alkaline phosphatase.

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蔡晓布,周进,钱成.不同退化程度高寒草原土壤微生物活性变化特征研究[J].土壤学报,2008,45(6):1110-1118. DOI:trxb10.11766/200706300614 Cai Xiaobu, Zhou Jin, Qian Cheng. VARIATION OF SOIL MICROBIAL ACTIVITIES IN ALPINE STEPPES DIFFERENT IN DEGRADATION INTENSITY IN THE NORTH TIBET PLATEAU[J]. Acta Pedologica Sinica,2008,45(6):1110-1118.

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  • 收稿日期:2007-06-30
  • 最后修改日期:2007-10-23
  • 在线发布日期: 2013-05-10
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