Abstract:A Trichoderma mutant strain T32, which can grow on the carbendazim-containing culture medium was isolated out of pesticide-tolerant Trichoderma strains. Its carbendazim degrading rate reached 61.4% in the mineral medium that utilizes carbendazim as the sole carbon source after 5 days of shake at temperature 25℃ and 200 r min-1. In the medium 6.0 in pH value, 25℃ in temperature, 5% in inoculation rate and 05% in yeast addition rate, its degrading rates of five common chemical pesticides — carbendazim, procymidone, iprodione, thiophanatemethyl and triadimefon reached 91.4%, 92.1%, 55.3%, 40.1% and 86.5% respectively, demonstrating that Strain T32 has a relatively extensive degradation capacity Trichoderma can degrade five different kinds of chemical pesticides including carbendazim at the same time, which has not yet been involved in the present research on degrading bacteria. An indoor test of degrading carbendazim in original soil, natural air-drying soil and high-temperature ovendrying soil for 10 days revealed that their carbendazim degrading rate was 78.6%,75.3% and 70.5%, respectively, when the optimal degradation conditions were 25~28℃ in temperature, 5% in inoculation rate, 50 mg g-1in concentration of carbendazim and 15% in water content. The findings may serve as a scientific basis for applying Strain T32 to bioremediation of polluted soil.