通过根盒试验比较了籼稻汕优63和粳稻武运粳7号苗期不同采样期根际土和土体土壤的硝化强度以及氨氧化细菌数量的差异，并且采用16S rDNA PCR-DGGE（Denaturing gradient gel electrophoresis）指纹图谱技术比较分析了上述两种水稻苗期不同采样期根际和土体土壤中细菌及其氨氧化细菌的群落结构变化。结果表明，两个水稻品种根际土壤中硝化强度和氨氧化细菌的数量随着生育期的延长均表现出一定的正相关性，汕优63籼稻根部土壤中的细菌和氨氧化细菌的丰富度和群落变化特征随着水稻生育时期的延长较武运7号粳稻的变化更为多样，说明籼稻品种根系和根际硝化作用更强，在其根系附近会产生更多的硝态氮。这种差异性严重影响水稻植株对氮素的利用效率．
Significant difference of nitrification can be always found between rices different in cultivar, which may result in difference in nitrogen use efficiency between the two rice cultivars. A rhizobox with three compartments and subsequent soil-slicing was used to determine simultaneously variation of nitrification and ammonia oxidizing bacteria (AOB) in the rhizospheric soils of two rice cultivars, i.e. Shanyou 63 (Indica) and Wuyun 7 (Japonica), growing at an N level of 30 mg kg-1 soil -1. Composition of AOB communities in the rhizosphere was studied by means of DGGE (denaturing gradient gel electrophoresis) of 16S rDNA of the AOB. Results show that nitrification activities of both rice cultivars increased with the incubation time. Peak nitrification activities were found in rhizospheric soil. In the rhizosphere nitrification activities decreased with the distance from the root. The maximum nitrification activity was measured to be at 0.77 mg kg -1 h-1 and 0.73 mg kg -1 h -1, in Shanyou and Wuyun respectively. The populations of AOB in the root zone, rhizosphere (0～4 mm away from the root zone) and bulk soil (>4 mm away from the root zone) for both rice cultivars increased during the growth periods The composition of AOB communities in the rhizosphere of Shanyou 63 is more abundant than of Wuyuny 7, showing that Indica might have a stronger nitrification in rhizosphere and on root surface and hence have a higher capability of utilizing nitrate than Japonica.
赵爽,胡江,沈其荣.两个水稻品种根际土壤细菌和氨氧化细菌的群落结构差异[J].土壤学报,2010,47(5):939-945. DOI:10.11766/trxb200811260516 Zhao Shuang, Hu Jiang, Shen Qirong. Difference in community structure of bacteria and ammonia-oxidizing bacteria in rhizosphere between two different rice cultivars[J]. Acta Pedologica Sinica,2010,47(5):939-945.复制