枯草芽孢杆菌Tu-100对油菜根系分泌物所含氨基酸的趋化性研究
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国家自然科学基金项目(30870451)、国家农业产业技术体系项目(nycytx-005)和“十一五”国家科技支撑计划项目(2006BAD21B03) 资助


Chemotaxis of bacillus subtilis TU-100 toward amino acids in root exudates of oilseed rape
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    摘要:

    应用水培的方法采集油菜根系分泌物;采用高效液相色谱测定出油菜根系分泌物中所含的16种氨基酸;使用烧杯法和平板趋化法进行趋化性试验,分别测定枯草芽孢杆菌Tu-100对16种氨基酸、油菜根系和油菜菌核的趋化性。结果表明Tu-100仅对组氨酸、脯氨酸、精氨酸、天门冬氨酸和谷氨酸等5种氨基酸有趋化性,其中组氨酸的趋化性最强;Tu-100对油菜根系和油菜菌核也有趋化性,对油菜根系的趋化性要大于油菜菌核。

    Abstract:

    Root exudates of oilseed rape were collected from the plants in water culture, and determined to contain 16 kinds of amino acids with high performance liquid chromatography (HPLC). Chemotaxis of Bacillus subtilis Tu-100 toward the 16 amino acids, oilseed rape roots and sclerotia, were determined separately with the beaker method and petri dish method. Results show that Tu-100 displayed obvioous chemotaxis toward histidine, proline, arginine, aspartic acid and glutamic acid only, and histidine in particular, and toward oilseed rape roots and sclerotia as well; more obvious toward the former than the latter.

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胡小加,余常兵,李银水,刘胜毅,廖星.枯草芽孢杆菌Tu-100对油菜根系分泌物所含氨基酸的趋化性研究[J].土壤学报,2010,47(6):1243-1248. DOI:10.11766/trxb200908070341 Hu Xiaojia, Yu Changbing, Li Yinshui, Liu Shengyi, Liao Xing. Chemotaxis of bacillus subtilis TU-100 toward amino acids in root exudates of oilseed rape[J]. Acta Pedologica Sinica,2010,47(6):1243-1248.

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  • 收稿日期:2009-08-07
  • 最后修改日期:2010-06-18
  • 录用日期:2010-08-17
  • 在线发布日期: 2010-08-31
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