Contamination of phthalate esters (PAEs) in agricultural soils is reported frequently in China recently and has raised wide concern to researches due to the effects of endocrine disrupt. Ultrasonic extraction and Soxhlet extraction were both performed to extract the added PAEs from soil. The results indicated that the ultrasonic extraction was more feasible to be the pretreatment method for PAEs analysis of soil sample as a result of increasing recovery and decreasing procedure blank. Meanwhile, operating procedures of ultrasonic extraction were studied further, Preservation of 1-2ml of the extract instead of dryness will effectively prevent the loss of compounds from volatilization in the process of rotating evaporator. No obvious difference was observed between the treatment of cleanup and without cleanup by florisil when analyzed by GC-MS in present study. Skip of the cleanup process will increase the efficiency of PAEs analysis, however, the interference of pigment or other components in the soil samples will have an impact on the accuracy of Bis(2-ethylhexyl) phthalate (DEHP) and other target compouds.