产漆酶真菌筛选及其对PAHs污染土壤修复的初步研究
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国际科学基金项目及南京师范大学“211工程”三期重点学科建设项目


Screening for laccase-producing fungus and its potential in remediation of PAHs-contaminated soil
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    摘要:

    真菌漆酶可以高效转化多环芳烃(PAHs),因此,产漆酶真菌在PAHs污染土壤修复中极具应用前景。根据漆酶可将愈创木酚氧化为红色物质的特性,成功从土壤中筛选出一株能够分泌漆酶的真菌菌株F-1,初步鉴定该菌为疣孢漆斑菌(Myrothecium verrucaria)。通过Plackett-Burman试验对菌株F-1的产酶能力进行了分析,发现特定培养条件组合可将其酶活提高近300倍,达5 628 U L-1,表明F-1的漆酶活性受到环境条件的显著影响。应用菌株F-1对PAHs污染土壤进行了初步修复研究,结果表明,接种F-1对菲、荧蒽、芘、苯并(a)蒽、屈、苯并(b)荧蒽、苯并(k)荧蒽、苯并(a)芘、二苯并(a,h)蒽、苯并(g,h,i)苝、茚苯(1,2,3-cd)芘等11种PAHs均有不同程度的降解,提示产漆酶真菌在PAHs污染土壤修复中的应用潜力。

    Abstract:

    Fungal laccase can efficiently oxidize polycyclic aromatic hydrocarbons (PAHs), suggesting the potential of laccase-producing fungi for application to remediation of PAHs-contaminated soil. Based on its feature of being capable of oxidizing guaiacol into something red, a strain of laccase-producing fungus, F-1, was isolated from soil. According the BLAST alignment of near full-length 18S rRNA gene sequence, this strain is closely related to Myrothecium verrucaria. Both single factor and Plackett-Burman experiments were performed to assess F-1’s laccase-producing capacity. The laccase activity was found to be greatly increased by 2 orders of magnitude in specific culture medium, suggesting the significant effect of environment factors on laccase activity of F-1. Soil microcosms were set up with or without inoculation of F-1, and the PAHs contents were determined after 30-days of incubation. Results show that PhA, FluA, Pyr, BaA, Chr, BbF, BkF, BaP, DbA, BghiP and In[1,2,3-cd]P were degraded to varying extent in fungus inoculated microcosms, confirming the remedial potential of F-1 in PAHs-contaminated soil.

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潘澄,茆婷,吴宇澄,申卫收,钟文辉.产漆酶真菌筛选及其对PAHs污染土壤修复的初步研究[J].土壤学报,2011,48(6):1253-1259. DOI:10.11766/trxb201005250209 PAN CHENG, MAO TING, WU YUCHENG, SHEN WEISHOU, ZHONG WENHUI. Screening for laccase-producing fungus and its potential in remediation of PAHs-contaminated soil[J]. Acta Pedologica Sinica,2011,48(6):1253-1259.

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  • 收稿日期:2010-05-25
  • 最后修改日期:2011-01-11
  • 录用日期:2011-03-07
  • 在线发布日期: 2011-09-02
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