Soil organic carbon and total nitrogen are of crucial importance to soil fertility, crop yield, agricultural sustainable development and global carbon and nitrogen cycling. To evaluate effects of tillage and rotation on storages of soil organic carbon (SOC) and total nitrogen (TN) in a black soil in Northeast China, an 8-year stationary field experiment was conducted in a black soil field in Dehui, Jilin. The experiment was designed to have three tillage treatments and two cropping treatment, i.e. no-tillage (NT), ridge tillage (RT) and moldboard plow (MP), and corn-soybean rotation (CS) and continuous corn (CC). Variation of SOC and TN in various soil layers were monitored by sampling and calculated using the method of equivalent mass for soil organic matter. Results show that under the CS system, both SOC and TN decreased somewhat in Treatment MP, but significantly increased in Treatment NT in the 0-5cm soil layer. However, the increase in SOC in NT was offset by the loss of SOC in the sub-surface layer, leaving the stock of SOC unchanged in the soil. Treatment RT significantly increased SOC and TN in the 0-5 cm and 5-10 cm layers, or by 4.9% and 10.7%, respectively, in the 0-30 cm soil layer. Under the CC system, SOC and TN stocks increased in Treatments MP and NT, and the increase in Treatment NT was 3.2 times as high as that under the CS system. C/N ratios in all the treatments declined, and particularly in Treatment RT, it dropped form 12.05 to 11.04 in the 0～5 cm soil layer, which was 3.2 and 2.8 times as great as in Treatments NT and MP, respectively, in magnitude of the drop. It is learnt from the above findings that neither MP nor NT is efficient in promoting SOC sequestration under corn-soybean rotation in black soil with a mid-layer heavy and clayey in texture and poor in drainage whereas RT promotes SOC and TN accumulation, which is not only conducive to improving soil fertility, but also effective to turn farmland soil from source to sink for CO2. Compared with the corn-soybean rotation system, continuous corn cropping is more favorable to SOC and TN sequestration.