PGPR bacterial manure is getting more and more popular in crop and vegetable production, but it has not yet been specialized enough for use in flue-cured tobacco production. In this study, the PGPR bacterial manure used was prepared out of the antibiotic bacteria, nitrogen fixing bacteria, phosphate-dissolving bacteria, and potassium-dissolving bactera screened and isolated from flue-cured tobacco rhizosphere. The experiment was laid out in an experiment field of yellow brown soil, using Yuanyan 89, a common tobacco cultivar. The application rate of the PGPR bacterial manure was set at 30 kg hm^-2 when tobacco seedlings was transplanted to the plots randomly arranged. The experiment had four treatments, viz., Treatment I, 100% NPK + PGPR (conventional NPK application rate for tobacco + PGPR bacterial manure), Treatment II, 80% NPK + PGPR (80% the conventional NPK application rate + PGPR), Treatment III, 100% NPK (Conventional NPK application rate) and Treatment IV, 80% NPK (80% of the conventional NPK application rate). Results show that in the PGPR treatments the count of actinomyces in tobacco rhizospheres was reduced by 17% to 27% (p＜0.05), while the content of microbial biomass carbon increased by 3% to 16% during the whole growth period; the count of phosphate-dissolving bacteria at the budding stage increased by 24% (p＜0.05); and the quality of cured leaves was better, as compared to those in the non-PGPR treatments. Comparison between Treatment II and Treatment IV revealed that application of PGPR increased the availability of N, K, Cu, Zn, B, and Fe in tobacco rhizosphere at budding stage by 4.46%~28.87%, while decreased the availability of P, Ca, Mg, and Mn by 2.63%~30.19%, however, it improved yield and net production value of the flue-cured tobacco by 7.53% and 30.05%%, respectively. Compared with Treatment III, Treatment II increased yield and net production value of the flue-cured tobacco by 4.52% and 24.68%. The findings suggest that the use of PGPR bacterial manure is an effective approach to reduction of the application rate of chemical fertilizers in tobacco production and sustainable production of quality and hazard-free flue-cured tobacco leaves.