Schwertmannite, jarosite and goethite are common secondary iron minerals found in acid mining drainage. They were formed biological under normal temperature and pressure with the aid of Acidithiobacillus ferrooxidans in this study. Batch adsorption experiments were conducted under three different temperatures (15 ^℃, 25 ^℃ and 35 ^℃) to explore arsenite adsorption behaviors of the three biogenic secondary iron minerals in simulated acid mining drainage (pH3.0). It was found that arsenite adsorption of the three biogenic minerals were of the second order of reaction and could well be described by the Lagergren pseudo-second order rate equation, with correlation coefficient being < 0.97. Their adsorption rate constant was 0.094 g mg^-1min^-1 for schertmannite, 0.042 g mg^-1min^-1 for goethite and 0.02 g mg^-1 min^-1 for jarosite, and their sorption isotherms fitted the Langmuir and Freundlich model with correlation coefficient being >0.94. Their adsorption enthalpy was 11.76, 18.40 and 9.34 kJ mol^-1, separately for the three different minerals and their Δ G’s were all <0. The adsorption of arsenite was a kind of endothermic spontaneous process.