N2O产生法测定土壤无机态氮15N丰度
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中国科学院南京土壤研究所所长基金(2007年)项目资助


Determining 15N abundance in ammonium, nitrate and nitrite in soil by measuring nitrous oxide produced
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    摘要:

    建立了一个测定土壤中微量铵、硝酸盐和亚硝酸盐的15N丰度的质谱分析方法。其原理是,用化学方法分别将它们转化为N2O气体,然后用带自动预浓缩装置的同位素比值质谱仪测定N2O中的15N丰度。N2O中的15N丰度测量值完全符合铵、硝酸盐和亚硝酸盐中的15N参考值。方法快速和简单,不受空气氮的污染。特别是方法的检测限低,每批次样品中只需含5~20μg N。它将有助于土壤氮素的矿化作用、硝化作用和反硝化作用的研究。

    Abstract:

    A new method was designed for direct determination of 15N abundance of NH4+, NO3- and NO2- in soils with mass spectrometry. The principle of this method was based on analysis and quantification of 15N abundance of the N2O produced from NH4+,NO3-and NO2- through transformation reactions catalyzed with their respective specific chemical reagents separately. The measured values of 15N abundance of the N2O tally well with their respective reference values in NH4+, NO3- and NO2-. The method was characterized by quickness, simpleness and freedom from contamination of atmospheric nitrogen. Especially this method has a very low detection limit, and 5~20 μg N in the sample is adequate for detection. Therefore, it will be of a great help to study on mineralization, nitrification and denitrification of nitrogen in soils.

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曹亚澄,钟 明,龚 华,陆国兴. N2O产生法测定土壤无机态氮15N丰度[J].土壤学报,2013,50(1):113-119. DOI:10.11766/trxb201204160136 Cao Yacheng, Zhong Ming, Gong Hua, Lu Guoxing. Determining 15N abundance in ammonium, nitrate and nitrite in soil by measuring nitrous oxide produced[J]. Acta Pedologica Sinica,2013,50(1):113-119.

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  • 收稿日期:2012-04-16
  • 最后修改日期:2012-10-16
  • 录用日期:2012-10-17
  • 在线发布日期: 2012-10-30
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