氮素富集对青藏高原高寒草甸土壤有机碳迁移和累积过程的影响
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农业部农业环境与气候变化重点开放实验室开放基金、中国科学院知识创新工程重要方向项目(Y225017EA2)和中国科学院地理科学与资源研究所“秉维”优秀青年人才基金(2011RC202)资助。


Effects of nitrogen enrichment on transfer and accumulation of soil organic carbon in alpine meadows on the Qinghai-Tibetan Plateau
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    摘要:

    为深入揭示陆地生态系统碳固定对大气氮沉降增加的响应机理,基于海北高寒草甸多形态(NH4Cl、(NH4)2SO4、KNO3)、低剂量(N 0、10、20、40 kg hm-2 a-1)的增氮控制试验平台,采集各处理水平下不同深度土壤样品,利用颗粒分组法分离测定总土壤有机碳(SOC)以及各粒径组分的碳含量和δ13C值。研究结果表明:低氮显著增加了土壤粗颗粒态有机碳(MacroPOC)和矿质结合态有机碳(MAOC)的含量,而高氮处理正好相反。施氮一致降低土壤细颗粒态有机碳(MicroPOC)含量。此外,添加硝态氮肥对SOC各组分含量和δ13C值的影响显著高于铵态氮肥。总体而言,低氮导致地表30 cm层SOC储量增加了4.5%,而中氮和高氮导致SOC储量分别下降了5.4%和8.8%。低氮处理时新增的碳以MacroPOC为主,而高氮处理时损失的碳主要是MicroPOC。连续5 a施氮促进了颗粒态有机碳(POC)组分的分解,进而导致SOC稳定组分的比例增加。可以认为,大气氮沉降或低剂量施氮(10 kg hm-2 a-1)短期内有利于青藏高原高寒草甸土壤碳截留,硝态氮较铵态氮输入对土壤碳储量增加更为有益。

    Abstract:

    Increasing atmospheric nitrogen (N) deposition can significantly change carbon (C) cycling rates and budget in the terrestrial ecosystem, and is generally considered to be an important pathway of missing of the sink. However, the contribution of atmospheric N deposition to C sequestration in the terrestrial ecosystem is controversial. It is, therefore, essential to accurately evaluate the effects of rate and type of N deposition on amount, composition and stability of soil organic carbon (SOC). Stable C isotope natural abundance (or δ13C) contains various information concerning C cycling processes, and hence makes it feasible to track SOC in its transfer, transformation and accumulation processes. The technique of Stable 13C natural abundance provides a way to characterize the dynamics of SOC with different turnover times. It is hypothesized in this study that N deposition increases biomass of plants and13C-depleted plant debris that leads to expansion of the fraction of coarse particle-sized SOC. Meanwhile, increased N deposition promotes activities of soil microbes, and increases emission of 13C-depleted CO2, thus leading to decrease in SOC content and accumulation of δ13C. Consequently, this study is mainly aimed at determination of the effects of rate and type of N deposition on contents of SOC and its various particle-size fractions in the soil and quantification of relative contributions of the changes in these fractions to the change in total under the condition of N accumulation. Through the above-described researches, it is expected a better in-depth knowledge could be obtained regarding mechanisms of the effects of N deposition on deposit and stability of SOC. For that end a controlled multi-form, low-rate N addition field experiment was conducted at the Haibei Alpine Meadow Ecosystem Research Station in 2007. The experiment was designed to have three types of N fertilizers, NH4Cl, (NH4)4SO4 and KNO4, and four N application rates: 0, 10, 20 and 40 kg hm-2 a-1, and three replicates for each treatment. Soil samples, 10 in each treatment lot,were collected randomly with augers at 10cm intervals to a depth of 30 cm. A total of 1080 samples were collected in 2011 for determination of content and δ13C value of dissolved organic carbon, as well as contents of the three fractions of SOC: macro particle-sized organic carbon (MacroPOC, >250 μm), micro particle-sized organic carbon (MicroPOC, 53~250 μm) and mineral bonded organic carbon (MAOC, <53 μm). It was found that low N input significantly increased the contents of soil MacroPOC and MAOC, while high N input acted reversely; and N input, no matter high or low, decreased the content of MicroPOC. Besides, the effect of nitrate fertilizer was more significant than that of ammonium fertilizer. In short, For the entire soil profile, low N input increased the SOC storage in the 0~30cm soil layer by 4.5%, while medium or high N input decreased it by 5.4% or 8.8%, respectively. In the treatments of low N input, the increment of C consisted mainly of MicroPOC, while in the treatments of high N input, the decrement did mainly of MicroPOC, too. N application for 5 years in a row significantly promoted decomposition of the POC fraction of SOC, thus leading to increase in the proportion of stable fractions of SOC. The above-described findings indicate that N input may affect, increase or decrease, SOC storage in the alpine meadow, with the critical rate being set at 20 kg hm-2 a-1 while taking into account the ambient N deposition rate of 10 kg hm-2 a-1. Therefore, as long as the exogenous N input level is kept lower than this critical value, it is safe and beneficial to SOM accumulation in the alpine meadow on the Qinghai-Tibetan Plateau in the current period of time. These findings may serve as a theoretical basis for C and N management of alpine meadows in the future. Further study is still needed to explore microbial mechanisms of N input affecting SOC in quality and quantity.

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李林森,程淑兰,方华军,于贵瑞,徐敏杰,王永生,党旭升,李英年.氮素富集对青藏高原高寒草甸土壤有机碳迁移和累积过程的影响[J].土壤学报,2015,52(1):183-193. DOI:10.11766/trxb201404090159 Li Linsen, Cheng Shulan, Fang Huajun, Yu Guirui, Xu Minjie, Wang Yongsheng, Dang Xusheng, Li Yingnian. Effects of nitrogen enrichment on transfer and accumulation of soil organic carbon in alpine meadows on the Qinghai-Tibetan Plateau[J]. Acta Pedologica Sinica,2015,52(1):183-193.

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  • 收稿日期:2014-04-09
  • 最后修改日期:2014-07-02
  • 录用日期:2014-07-31
  • 在线发布日期: 2014-08-26
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