Supported by the National Natural Science Foundation of China (Nos. 31360507, 31560586 and 31460551)
采用盆栽试验，研究了肉桂酸不同浓度（C0（0.0 mg kg-1）、C1（14.0 mg kg-1）、C2（28.0 mg kg-1）和C3（56.0 mg kg-1））处理对蚕豆枯萎病发生的影响及小麦与蚕豆间作对肉桂酸自毒效应的缓解机制。结果表明：（1）蚕豆单作条件下，肉桂酸处理显著降低了根系的过氧化物酶（POD）活性并增加了丙二醛（MDA）含量，导致根系脂质过氧化伤害加剧；肉桂酸处理降低了根际微生物的活性（AWCD值）、香农（Shannon）多样性指数（H）和丰富度指数（S）并改变了微生物群落结构；肉桂酸处理显著增加蚕豆根际土壤中镰刀菌数量，提高了枯萎病病情指数并降低根系干重。（2）肉桂酸胁迫下，与单作蚕豆相比，小麦与蚕豆间作提高了蚕豆根系的POD活性，降低了MDA含量，提高了根际微生物对碳源的利用能力及根际微生物的H和S，明显改变了根际微生物群落结构；间作显著降低了蚕豆根际土壤中镰刀菌数量及枯萎病病情指数，显著增加了蚕豆地上部干重和根系干重。表明小麦与蚕豆间作一方面提高蚕豆对枯萎病菌的生理抗性，另一方面有效调节根际微生物活性和多样性，从而缓解了肉桂酸引起的枯萎病危害，促进了蚕豆生长，但间作减轻枯萎病危害的效果受肉桂酸处理浓度的影响。因此，利用非寄主作物小麦的化感效应而采取的间作模式是缓解蚕豆自毒效应的有效措施。
【Objective】The aim of this work is to investigate 1）whether the amendment of cinnamic acid could mitigate the incidence of Fusarium wilt and 2）how wheat and faba bean intercropping alleviates cinnamic acid autotoxicity. 【Method】A pot experiment was conducted on wheat and faba bean intercropping and amendment of cinnamic acid at a varying rate (C0（0.0 mg kg-1），C1（14.0 mg kg-1），C2（28.0 mg kg-1） and C3（56.0 mg kg-1）). 【Result】1) In comparison with Treatment C0, amendment of cinnamic acid decreased the activity of peroxidase (POD), but significantly increased the content of malondialdehyde (MDA), thus leading to disorder of metabolic and physiological functions and aggravation of the damage of membrane lipid by peroxidation in the treatments under monocropping of faba bean．2) The amendment of cinn-amic acid decreased the average well color development (AWCD), Shannon-Wiener diversity index (H) and substrate richness (S), thus altering soil microbe community structure in faba bean rhizosphere, by increasing the number of Fusarium oxysporum; and increasing the incidence index of faba bean Fusarium wilt. As a result, dry weight of the root system of monocropped faba bean decreased. 3) Among the treatments amended with cinnamic acid at the rate of C1, C2 and C3, the treatments of intercropped faba bean, were 22.4%, 81.0% and 100.0% higher in POD activity of faba bean, and 45.1%, 92.4% and 145.1% lower in MDA content than their corresponding ones of monocropped faba bean. 4) Treatments C1 and C2 of intercropped faba bean were 110.5% and 83.9% higher in total carbon utilization ability than their corresponding ones of monocropped faba bean, especially in utilization of carbohydrates, amino acids, and carboxylic acids. Besides, they were also much higher in Shannon-Wiener diversity index and substrate richness, had soil microbe community structure in the rhizosphere significantly changed by decreasing the population of Fusarium oxysporum in the faba bean rhizosphere by 16.7%~20.2% and 41.1%~51.8%, and increased root dry weight by 11.3%~23.6% and 72.9%~154.9%, respectively. 【Conclusion】Intercropping of faba bean with wheat significantly increases physiological resistance of faba bean to Fusarium oxysporum f. fabae, and significantly lowers the incidence of Fusarium wilt on one hand and effectively regulates soil microbial activity and diversity in the faba bean rhizosphere soil, on the other, thus alleviating the harm of Fusarium wilt caused by cinnamic acid and promoting growth of the crop. However, the effect is affected by concentration of cinnamic acid. By making use of the allelopathic effect of wheat as non-host crop, the intercropping of faba bean with wheat is an effective measure to alleviate autotoxicity of cinnamic acid.
董 艳,董 坤,杨智仙,朱锦惠,汤 利,郑 毅.肉桂酸对蚕豆枯萎病发生的影响及间作缓解机制[J].土壤学报,2017,54(2):503-515. DOI:10.11766/trxb201605030043 DONG Yan, DONG Kun, YANG Zhixian, ZHU Jinhui, TANG Li, ZHENG Yi. Effect of Cinnamic Acid on Incidence of Faba Bean Fusarium Wilt and Incidence-Mitigating Mechanisms of Wheat and Faba Bean Intercropping[J]. Acta Pedologica Sinica,2017,54(2):503-515.复制