国家自然科学基金项目(30970499)和世界银行贷款项目 ( SEAP-KY-2) 共同资助
Supported by the National Natural Science Foundation of China (No. 30970499) and the World Bank Loan Project (No. SEAP-KY-2)
为精确分析黄河三角洲刺槐根际与非根际土壤细菌群落定殖情况，本研究采用高通量测序方法对刺槐根际与非根际土壤细菌结构及多样性进行了研究。研究表明，根际土壤细菌共有36门214属，非根际土壤细菌共有33门153属。变形菌门（Proteobacteria）、放线菌门（Actinobacteria）、酸杆菌门（Acidobacteria）细菌丰度超过15%，是根际与非根际土壤中的优势菌落。根际与非根际土壤中酸杆菌门、硝化螺旋菌门（Nitrospirae）丰度差异显著。根际土壤中红游动菌属（Rhodoplanes）、溶杆菌属（Lysobacter）、热单胞菌属（Thermomonas）、链霉菌属（Streptomyces）及非根际土壤中红游动菌属、溶杆菌属、链霉菌属、Kaistobacter细菌丰度超过4%。根际土壤中固氮菌丰度显著高于非根际土壤，解磷、解钾细菌丰度差异不显著。根际与非根际土壤细菌Chao丰富度分别为2 054、2 376，差异显著。根际与非根际土壤细菌之间的权重（Weighted Unifrac）距离在0.12～0.25之间。综上所述，黄河三角洲刺槐根际与非根际土壤细菌结构具有一定差异，多样性差异显著。
【Objective】 As one of the three major pedogenic river deltas in China and the one that develops the fastest, the Yellow River Delta is an important reserved land resource. Developing mixed forests has been becoming a major strategy to develop the Yellow River Delta，because mixed forests can not only regulate temperature but also improve soil fertility. To improve the ecological system of the Yellow River Delta, a number of forest plantations have been established since the 1950s. Robinia pseudoacacia is the dominant tree species in most of the forest plantations, which contribute significantly to conserving soil and water and improving the ecological system of the Delta. Soil microbial community in plant rhizosphere plays an important role in plant nutrition and plant health. This research aimed to accurately evaluate how soil bacterial communities colonize in rhizosphere and in bulk soils under Robinia pseudoacacia in the Yellow River Delta. 【Method】 With the aid of the Illumina MiSeq technology, this research analyzed structure and diversity of the bacterial communities in the rhizosphere soil and the bulk soil under Robinia pseudoacacia for comparison. The differences in soil bacterial community between rhizosphere and bulk soil were expressed via the Weighted Unifrac Range of OTUs. 【Result】Results show that the bacterial community in the rhizosphere soil consisted of 214 genera of 36 phyla and that in the bulk soil 153 genera of 33 phyla. Proteobacteria, Actinobacteria and Acidobacteria were the dominant phyla of bacteria in both rhizosphere and bulk soils with relative abundance being more than 15%. Acidobacteria and Nitrospirae varied sharply in relative abundance between the rhizosphere soil and the bulk soil. Chloroflexi, Gemmatimonadetes, Planctomycetes, Nitrospirae, and Verrucomicrobia were the dominant taxa of bacteria with relative abundance being more than 1%. Rhodoplanes, Lysobacter, Thermomonas and Streptomyces in the rhizosphere soil and Rhodoplanes,Lysobacter, Streptomyces and Kaistobacter in the bulk soil all exceeded 4% in relative abundance. There were 29 and 32 genera of bacteria in the rhizosphere soil and 3 in the bulk soil being more than 1% in relative abundance, respectively. Significant differences were found in relative abundance of Novosphingobium, Aeromicrobium, Limnohabitans, Phycicoccus, aistobacter, Candidatus Nitrososphaera, DA101, Ramlibacter, and Methylibium between the rhizosphere soil and the bulk soil. Relative abundance of Azotobacter was significantly higher in the rhizosphere soil than in the bulk soil, while no significant difference was observed in relative abundance of potassium and phosphorus-dissolving bacteria between the two soils. Chao richness of bacterial community was 2 054 and 2 376 in the rhizosphere soil and in the bulk soils, respectively, showing a significant difference. Weighted Unifrac Range mof the soil bacteria between the rhizosphere and the bulk soil was 0.12～0.25. In this study, all the findings demonstrate that there are some differences in structure between the bacterial communities in the rhizosphere soil and in the bulk soil under Robinia pseudoacacia in Yellow River Delta. 【Conclusion】Significant differences do exist in relative abundance of Proteobacteria, Acidobacteria, and Nitrospirae at the phylum level, and Novosphingobium, Aeromicrobium, Limnohabitans, andPhycicoccus at the genus level between rhizosphere and bulk soils, and significant differences are also observed in bacterial diversity between rhizosphere and bulk soils. Analysis of bacteria 16S rRNA-bacteria dataset shows differernces in structure and diversity of soil bacterial community between the rhizosphere and bulk soila in the Yellow River Delta. But further research is still needed to get more detailed information about soil microorganisms.
丁新景,敬如岩,黄雅丽,陈博杰,马风云.黄河三角洲刺槐根际与非根际细菌结构及多样性[J].土壤学报,2017,54(5):1293-1302. DOI:10.11766/trxb201703230510 DING Xinjing, JING Ruyan, HUANG Yali, CHEN Bojie, MA Fengyun. Bacterial Structure and Diversity of Rhizosphere and Bulk Soil of Robinia Pseudoacacia Forests in Yellow River Delta[J]. Acta Pedologica Sinica,2017,54(5):1293-1302.复制