基于微根管技术的盐胁迫下小麦根系生长原位监测方法
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中图分类号:

S127;S151.9

基金项目:

国家重点研发计划项目(2018YFC1800104-02,2018YFD0301301)、中国农业科学院农田灌溉研究所开放课题(FIRI2018-07-02)资助


In-situ Monitoring Method of Wheat Root Growth under Salt Stress Using Minirhizotron Technique
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Fund Project:

Supported by the National Key Research and Development Program of China (Nos. 2018YFC1800104-02 and 2018YFD0301301) and the Open Project of Farmland Irrigation Institute of Chinese Academy of Agricultural Sciences (No. FIRI2018-07-02)

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    摘要:

    常用的作物根系生长监测一般采用破坏性采样方法,如土钻法和挖掘法等,虽然精度较高,但很难实现对作物根系生长的原位重复观测。采用桶栽法,利用微根管技术对分蘖期、返青期、拔节期和孕穗期的小麦根系进行了连续观测和采样,获取不同盐胁迫下小麦根长密度和根长等参数,研究不同生长时期小麦根系生长参数随土壤深度分布的规律。结果显示,微根管法获得的小麦根长密度与土钻法所得到的结果呈极显著正相关(r=0.91),且在拔节期和孕穗期二者的相关性最好。通过不同时期根系图像对比及根系参数分析发现,小麦根系在0~10 cm土层分布最多,并随深度增加而减少。此外,随着土壤盐含量的增加,各生长期根长变短;在分蘖期,土壤盐分含量最高(S5,盐分含量6.61 g·kg-1)的小麦根系长度不足对照处理的1/2,至孕穗期,其根系长度甚至低至对照处理的1/3,说明小麦根系受盐分胁迫影响较大,且以孕穗期受胁迫程度最严重,尤其当土壤盐含量超过3 g·kg-1时影响最明显。由此可见,与传统破坏性取样方法相比,微根管技术结合图像处理技术可更好地快速、无损获取小麦根系生长的相关参数,为盐渍化区域作物根系的原位观测研究提供了新的方法。

    Abstract:

    [Objective] Generally, root is the main organ of a plant to absorb water and nutrients in soil, and how it grows and distributes is an important indicator of a crop in growth and development. The commonly used methods for monitoring crop root growth, such as the soil drilling and excavation methods, are what we call destructive sampling methods. Although they are usually quite high in accuracy, they are not suitable for in situ monitoring of root growth. Therefore, the minirhizotron technique is recommended as an effective method for in-situ monitoring of crop root growth thanks to its rapid and non-destructive approach.[Method] In order to reveal impacts of salt stress on root growth of wheat, a pot culture experiment was conducted, with soil salt content varying along a gradient from 0.61(CK), 1.61(S1), 2.61(S2), 3.61(S3), 4.61 (S4) to 5.61 g·kg-1 (S5). And the minirhizotron technique was used to- collect digital images of the roots at tillering, reviving, jointing and booting growing stages. Then root length, root length density and their distribution in the soil as affected by salt stress were determined with the aid of the digital image processing technology.[Result] Results show that with the aid of the minirhizotron technique, growth and development progresses of the wheat root in the experiment could be intuitively monitored. In the experiment the root length density obtained with the technique was found to be significantly and positively related with that with the soil drilling method (r=0.91), especially at the jointing and booting stages of the crop. Moreover, comparisons of the root images with the root parameters analysis relative to growing stage of the crop revealed that wheat root distributed mostly in the 0-10 cm soil layer and decreased with soil depth, and that root length decreased with rising soil salt content at all growing stages. For example, at the tillering stage, the root length in treatment S5 (S5, 6.61 g·kg-1) was less than half of that in CK, and at the booting stage it was only one-third of that in CK, indicating that the wheat root growth was greatly inhibited by salt stress. The worst occurred at the booting stage, especially in the treatments with soil salt content higher than 3 g·kg-1. It was mainly because high soil salt content caused the root rust and dead, and the higher the soil salt content was, the more obvious this phenomenon was.[Conclusion] Thus, the minirhizotron technique combined with the image processing technique could be used as a more effective method for obtaining root growth parameters as compared with traditional destructive sampling methods. This paper provides a theoretical basis and technical support for in situ monitoring intuitively of crop root growth in saline soils.

    参考文献
    [1] Parida A K,Das A B. Salt tolerance and salinity effects on plants:A review[J]. Ecotoxicology and Environmental Safety,2005,60(3):324-349.
    [2] Xie W J,Zhang Y P,Zhang M,et al. Relationships between soil physicochemical properties and wheat production in coastal saline soil[J]. Acta Pedologica Sinica,2015,52(2):461-466.[谢文军,张衍鹏,张淼,等. 滨海盐渍化土壤理化性质与小麦生产间的关系[J]. 土壤学报,2015,52(2):461-466.]
    [3] Smith T E,Grattan S R,Grieve C M,et al. pH dependent salinity-boron interactions impact yield,biomass,evapotranspiration and boron uptake in broccoli(Brassica oleracea L.)[J]. Plant and Soil,2013,370(1/2):541-554.
    [4] Yao R J,Yang J S,Wu D H,et al. Evaluation of pedotransfer functions for estimating saturated hydraulic conductivity in coastal salt-affected mud farmland[J]. Journal of Soils and Sediments,2015,15(4):902-916.
    [5] Thorup-Kristensen K,Dresbøll D B,Kristensen H L. Crop yield,root growth,and nutrient dynamics in a conventional and three organic cropping systems with different levels of external inputs and N re-cycling through fertility building crops[J]. European Journal of Agronomy,2012,37(1):66-82.
    [6] Palta J A,Yang J C. Crop root system behaviour and yield[J]. Field Crops Research,2014,165:1-4.
    [7] Maeght J L,Rewald B,Pierret A. How to study deep roots-and why it matters[J]. Frontiers in Plant Science,2013,4:299.
    [8] Amato M,Lupo F,Bitella G,et al. A high quality low-cost digital microscope minirhizotron system[J]. Computers and Electronics in Agriculture,2012,80:50-53.
    [9] Box J E,Ramsuer E L. Minirhizotron wheat root data:Comparisons to soil core root data[J]. Agronomy Journal,1993,85(5):1058-1060.
    [10] Gray S B,Strellner R S,Puthuval K K,et al. Minirhizotron imaging reveals that nodulation of field-grown soybean is enhanced by free-air CO2 enrichment only when combined with drought stress[J]. Functional Plant Biology,2013,40(2):13-147.
    [11] Taylor B N,Beidler K V,Strand A E,et al. Improved scaling of minirhizotron data using an empirically-derived depth of field and correcting for the underestimation of root diameters[J]. Plant and Soil,2014,374(1/2):941-948.
    [12] Rowland D L,Smith C,Cook A M,et al. Visualization of peanut nodules and seasonal nodulation pattern in different tillage systems using a minirhizotron system[J]. Peanut Science,2015,42(1):1-10.
    [13] Balogianni V G,Wilson S D,Farrell R E,et al. Rapid root decomposition decouples root length from increased soil C following grassland invasion[J]. Ecosystems,2015,18(8):1307-1318.
    [14] Bonin C L,Flores J P C,Lal R,et al. Root characteristics of perennial warm-season grasslands managed for grazing and biomass production[J]. Agronomy,2013,3(3):508-523.
    [15] Arnone J A,Zaller J G. Earthworm effects on native grassland root system dynamics under natural and increased rainfall[J]. Frontiers in Plant Science,2014,5:152.
    [16] Tierney G L,Fahey T J. Fine root turnover in a northern hardwood forest:A direct comparison of the radiocarbon and minirhizotron methods[J]. Canadian Journal of Forest Research,2002,32(9):1692-1697.
    [17] Tierney G L,Fahey T J. Evaluating minirhizotron estimates of fine root longevity and production in the forest floor of a temperate broadleaf forest[J]. Plant and Soil,2001,229(2):167-176.
    [18] Herrera J M,Noulas C,Feil B,et al. Nitrogen and genotype effects on root growth and root survivorship of spring wheat[J]. Journal of Plant Nutrition and Soil Science,2013,176(4):561-571.
    [19] Rose D,Ghamarnia H M,Gowing J W. Development and performance of wheat roots above shallow saline groundwater[J]. Soil Research,2010,48(8):659-667.
    [20] Liao R W,Liu J M,An S Q,et al. Monitor of corn root growth in soil based on minirhizotron technique[J]. Transactions of the Chinese Society of Agricultural Engineering,2010,26(10):156-161.[廖荣伟,刘晶淼,安顺清,等. 基于微根管技术的玉米根系生长监测[J]. 农业工程学报,2010,26(10):156-161.]
    [21] Yang J S. Development and prospect of the research on salt-affected soils in China[J]. Acta Pedologica Sinica,2008,45(5):837-845.[杨劲松. 中国盐渍土研究的发展历程与展望[J]. 土壤学报,2008,45(5):837-845.]
    [22] Wang Z Q,Zhu S Q,Yu R P. Salt-affected soil of China[M]. Beijing:Science Press,1993.[王遵亲,祝寿泉,俞仁培. 中国盐渍土[M]. 北京:科学出版社,1993.]
    [23] Pan X Z,Li Y L. A double tube observation system for plant root in situ:CN 205679254 U[P]. 2016-11-09.[潘贤章,李燕丽. 双筒式植物根系原位观测系统装置:CN 205679254 U[P]. 2016-11-09.]
    [24] Bai W M,Cheng W X,Li L H. Applications of minirhizotron techniques to root ecology research[J]. Acta Ecologica Sinica,2005,25(11):3076-3081.[白文明,程维信,李凌浩. 微根窗技术及其在植物根系研究中的应用[J]. 生态学报,2005,25(11):3076-3081.]
    [25] Merrill S D,Upchurch D R. Converting root numbers observed at minirhizotrons to equivalent root length density[J]. Soil Science Society of America Journal,1994,58(4):1061-1067.
    [26] Huang T X,Wang X G,Wu H,et al. Effects of drought and waterlogging stress on root growth of cotton[J]. Chinese Journal of Ecology,2018,37(7):2020-2029.[黄韬幸,王修贵,吴灏,等. 旱涝胁迫对棉花根系生长的影响[J]. 生态学杂志,2018,37(7):2020-2029.]
    [27] Wen P,Chen X B,Zhang L L,et al. Effects of salt and drought on winter wheat in seedling stage under different nitrogen rates[J]. Soils,2019,51(2):324-329.[文佩,陈小兵,张乐乐,等. 盐旱交叉胁迫对各施氮水平下小麦苗期的影响[J]. 土壤,2019,51(2):324-329.]
    [28] Zhang Q,He M R,Chen W F,et al. Effects of extraneous nitric oxide and salicylic acid on physiological properties of wheat seedlings under salt stress[J]. Acta Pedologica Sinica,2018,55(5):1254-1263.[张倩,贺明荣,陈为峰,等. 外源-氧化氮与水杨酸对盐胁迫下小麦幼苗生理特性的影响[J]. 土壤学报,2018,55(5):1254-1263.]
    [29] Wang S F,Hu Y X,Sun H J,et al. Effects of salt stress on growth and root development of two oak seedlings[J]. Acta Ecologica Sinica,2014,34(4):1021-1029.[王树凤,胡韵雪,孙海菁,等. 盐胁迫对2种栎树苗期生长和根系生长发育的影响[J]. 生态学报,2014,34(4):1021-1029.]
    [30] Miao G Y,Zhang Y T,Yin J,et al. A study on the development of root system in winter wheat under unirrigated conditions in semi-arid loess plateau[J]. Acta Agronomica Sinica,1989,15(2):104-115.[苗果园,张云亭,尹钧,等. 黄土高原旱地冬小麦根系生长规律的研究[J]. 作物学报,1989,15(2):104-115.]
    [31] Wang T,Rostamza M,Song Z H,et al. SegRoot:A high throughput segmentation method for root image analysis[J]. Computers and Electronics in Agriculture,2019,162:845-854.
    [32] Shi J W,Yu S Q,Yu L Z,et al. Application of minirhizotron in fine root studies[J]. Chinese Journal of Applied Ecology,2006,17(4):715-719.[史建伟,于水强,于立忠,等. 微根管在细根研究中的应用[J]. 应用生态学报,2006,17(4):715-719.]
    [33] Niu X L,Nan Z B. Review of minirhizotron applications for study of fine roots in grassland[J]. Acta Prataculturae Sinica,2017,26(11):205-215.[牛学礼,南志标. 运用微根管技术研究草地植物细根的进展[J]. 草业学报,2017,26(11):205-215.]
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李燕丽,王昌昆,卢碧林,李继福,潘贤章.基于微根管技术的盐胁迫下小麦根系生长原位监测方法[J].土壤学报,2021,58(3):599-609. DOI:10.11766/trxb201912310561 LI Yanli, WANG Changkun, LU Bilin, LI Jifu, PAN Xianzhang. In-situ Monitoring Method of Wheat Root Growth under Salt Stress Using Minirhizotron Technique[J]. Acta Pedologica Sinica,2021,58(3):599-609.

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  • 收稿日期:2019-12-31
  • 最后修改日期:2020-06-28
  • 录用日期:2020-09-21
  • 在线发布日期: 2020-12-10
  • 出版日期: 2021-05-11
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