首页 > 过刊浏览>2021年第58卷第3期 >628-637. DOI:10.11766/trxb201912050570
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长期施肥对红壤稻田土壤微生物生物量和酶活性的影响
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中图分类号:

S154.2;S147.35

基金项目:

国家重点研发计划项目（2016YFD0300900）和国家自然科学基金项目（31560582，31601263）资助


Effect of Long-term Fertilization on Soil Microbial Biomass and Enzyme Activities in Reddish Paddy Soil
Author:
  • XIA Wenjian

    XIA Wenjian

    Institute of Soil Fertilizer and Resource Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture and Rural Affairs/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang 330200, China
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  • LIU Kailou

    LIU Kailou

    Jiangxi Red Soil Research Institute, Nanchang 330046, China
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  • ZHANG Lifang

    ZHANG Lifang

    Institute of Soil Fertilizer and Resource Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture and Rural Affairs/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang 330200, China
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  • LIU Jia

    LIU Jia

    Institute of Soil Fertilizer and Resource Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture and Rural Affairs/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang 330200, China
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  • YE Huicai

    YE Huicai

    Jiangxi Red Soil Research Institute, Nanchang 330046, China
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  • DENG Guoqiang

    DENG Guoqiang

    Institute of Soil Fertilizer and Resource Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture and Rural Affairs/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang 330200, China
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  • LI Daming

    LI Daming

    Jiangxi Red Soil Research Institute, Nanchang 330046, China
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  • LI Zuzhang

    LI Zuzhang

    Institute of Soil Fertilizer and Resource Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture and Rural Affairs/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang 330200, China
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  • WANG Ping

    WANG Ping

    Institute of Soil Fertilizer and Resource Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture and Rural Affairs/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang 330200, China
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  • LI Yao

    LI Yao

    Institute of Soil Fertilizer and Resource Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture and Rural Affairs/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang 330200, China
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  • YANG Chengchun

    YANG Chengchun

    Institute of Soil Fertilizer and Resource Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture and Rural Affairs/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang 330200, China
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  • PENG Chunrui

    PENG Chunrui

    Institute of Soil Fertilizer and Resource Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture and Rural Affairs/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang 330200, China
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  • CHEN Jin

    CHEN Jin

    Institute of Soil Fertilizer and Resource Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture and Rural Affairs/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang 330200, China
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Fund Project:

Supported by the National Basic Research Program of China(No. 2016YFD0300900) and the National Natural Science Foundation of China (Nos. 31560582 and 31601263)

  • 摘要
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    摘要:

    研究长期施肥下红壤双季稻田土壤胞外酶活性（EEAs）变化特征及其主要驱动因子，可为该地区稻田土壤培肥和合理施肥提供理论依据。选择持续了37 a的长期定位试验的不施肥（CK）、化肥（NPK）、高倍化肥（HNPK）和化肥有机肥配施（NPKM）4个处理，采用微孔板荧光法测定了土壤胞外酶活性，分析了土壤化学指标和土壤微生物生物量碳（MBC）和微生物生物量氮（MBN），并通过主成分分析和冗余分析探讨土壤胞外酶分布特征及其与土壤养分和微生物生物量碳氮的关系。研究结果显示，长期施肥提高了土壤养分含量和水稻产量；与CK处理相比，NPKM处理土壤MBC和MBN分别提高了60.2%和60.4%，土壤α-葡萄糖苷酶（AG）、β-葡萄糖苷酶（BG）、乙酰氨基葡萄糖苷酶（NAG）和酸性磷酸酶（ACP）活性分别提高12.7%、41.1%、36.2%和50.0%，酚氧化酶（POX）活性下降29.7%。红壤稻田土壤EEAs的变化主要由养分因子驱动，其中土壤全氮（TN）和MBC是关键的决定因子，分别解释了酶活性变异的34.3%和20.9%。化肥配施有机肥有利于土壤养分、微生物生物量和土壤胞外酶活性提高，是维持作物高产和提升土壤质量最优的施肥管理措施。

    Abstract:

    [Objective] It is very important to know how extracellular enzyme activities (EEAs) vary and what are their driving factors in reddish paddy soil under long-term fertilization. The knowledge may serve as a certain theoretical basis for improvement of soil fertility and rational fertilization in the region. To that end, this study was unfolded.[Method] Based on a long-term fertilization field experiment that was started in 1981 and designed to have four treatments, that is (CK, without fertilization), NPK (fertilizer N, P and K), HNPK (double dosage of fertilizer N, P and K), NPKM (NPK fertilizers plus organic manure), soil samples were collected separately in each plot of the treatments after the later rice was harvested in 2017 for analysis of microbial biomass and soil chemical properties. Six soil extracellular enzymes involved in C, N and P cycling, were investigated with the microplate fluorometric assay method. For exploration of the distribution characteristics of soil EEAs and their relationship with soil nutrients and microbial biomass carbon and nitrogen in the reddish paddy soil, principal component analysis (PCA) and redundancy analysis (RDA) were performed.[Result] Results show that long-term fertilization increased soil nutrient content and rice yield. Compared with no fertilization control (CK), treatment NPKM increased soil microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN) significantly or by 60.2% and 60.4%, respectively, and soil AG, BG, NAG and ACP activities by 12.7%, 41.1%, 36.2%, and 50.0%, respectively, but reduced POX activity by 29.7%. The variations of EEAs were closely related to soil nutrient factors, among which soil total nitrogen (TN) and MBC might be the key determinants, accounting for 34.3% and 20.9% of the variation of EEAs, respectively.[Conclusion] It can be seen that the combined application of chemical fertilizer and organic manure is beneficial to improvement of soil fertility, microbial biomass and soil extracellular enzyme activity, and considered as the best fertilization management practice to maintain crop yield and improve soil quality.

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引用本文

夏文建,柳开楼,张丽芳,刘 佳,叶会财,邓国强,李大明,李祖章,王 萍,李 瑶,杨成春,彭春瑞,陈金.长期施肥对红壤稻田土壤微生物生物量和酶活性的影响[J].土壤学报,2021,58(3):628-637. DOI:10.11766/trxb201912050570 XIA Wenjian, LIU Kailou, ZHANG Lifang, LIU Jia, YE Huicai, DENG Guoqiang, LI Daming, LI Zuzhang, WANG Ping, LI Yao, YANG Chengchun, PENG Chunrui, CHEN Jin. Effect of Long-term Fertilization on Soil Microbial Biomass and Enzyme Activities in Reddish Paddy Soil[J]. Acta Pedologica Sinica,2021,58(3):628-637.

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  • 收稿日期:2019-12-05
  • 最后修改日期:2020-03-10
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