盐胁迫对番茄种子萌发中多胺形态变化和抗氧化的影响
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Q945.78

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国家自然科学基金项目(31672141)、江苏省现代农业(蔬菜)产业技术体系-苏州推广示范基地项目(JATS[2019]364)和温州市科技计划项目(N20180001)共同资助


Effects of Salt Stress on Form of Polyamine and Antioxidation in Germinating Tomato Seed
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Supported by the National Natural Science Foundation of China (No. 31672141), the Modern Agriculture (vegetable) Industry Technology System of Jiangsu Province- the Extension Demonstration Base Program of Suzhou City of Jiangsu Province (No. JATS[2019]364) and the Science and Technology Program of Wenzhou City of Zhejiang Province (No. N20180001)

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    摘要:

    以番茄“合作903”为材料,研究25~200 mmol·L-1NaCl胁迫对其种子发芽、种子活力指数(SVI)、丙二醛(MDA)含量、保护酶活性、溶质积累和不同形态多胺含量的影响。结果表明:25、50 mmol·L-1NaCl处理7 d,不影响番茄种子发芽率,但显著降低SVI;随着盐处理浓度上升,发芽率和SVI均显著下降。分别以种子发芽率和SVI为因变量,NaCl处理浓度为自变量,种子萌发和萌发后幼苗建成的耐盐阈值分别为106.1 mmol·L-1NaCl(0.62%)和43.38 mmol·L-1NaCl(0.25%)。随着NaCl处理浓度上升,萌发种子MDA含量显著上升,保护酶活性、可溶性蛋白(SP)和可溶性糖(SS)均上升,游离态腐胺(fPut)、游离态亚精胺(fSpd)和游离态精胺(fSpm)、(fSpd+fSpm)/fPut均显著上升,束缚态腐胺(bPut)、束缚态亚精胺(bSpd)、束缚态精胺(bSpm)均显著增加,而(bSpd+bSpm)/bPut变化不明显。与对照相比,50 mmol·L-1NaCl处理下,番茄萌发种子结合态亚精胺(cSpd)、结合态精胺(cSpm)含量及(cSpd+cSpm)/cPut均极显著上升,随着NaCl处理浓度上升,cPut、cSpd、cSpm含量及(cSpd+cSpm)/cPut均逐渐下降。综上所述,盐胁迫延缓、抑制番茄种子萌发,对于萌发后幼苗的建成抑制作用更强。番茄种子萌发阶段对盐分具有一定的抗性,主要由于番茄萌发种子中多胺代谢在盐胁迫下呈现(fSpd+fSpm)/fPut比值的急剧上升、cSpd和cSpm含量的显著上升和束缚态各多胺的显著积累,同时SS和SP的积累随盐胁迫加剧显著积累,从而提高了萌发种子的抗氧化和渗透调节能力;但是在高盐(150 mmol·L-1NaCl)下,cSpd和cSpm的上升调控作用明显减弱,而束缚态各多胺、fSpd和fSpm的上升以及SS和SP积累调控作用仍在增强。

    Abstract:

    [Objective] Tomato is one of the fruit and vegetable crops cultivated with the highest economic benefits in open fields and greenhouses. It is of great necessity to explore salt tolerance of germinating tomato seeds and its mechanism, especially changes in polyamine metabolism in the seeds.[Method] In this study, Impacts of NaCl stress on seed germination percentage (SGP) and development of radical and hypocotyl, seed water content (SWC), seed vigor index (SVI), Malondialdehyde (MDA) content, protective enzyme activity, solute accumulation and contents of various forms of polyamine of/in the seeds of tomato, "Hezuo 903" in variety were studied.[Result] When the soil was treated with 25 or 50 mmol·L-1 NaCl for seven days, germination of tomato seeds was slightly delayed, but SGP was not affected, however, SVI of the tomato decreased significantly. With rising salt concentration in the treatment, both the SGP and SVI decreased significantly. With SGP and SVI cited as dependent variables, and NaCl concentration in the treatment as independent variable, salt tolerance threshold for seed germination and seedling establishment was 106.1 mmol·L-1 NaCl (0.62%) and 43.38 mmol·L-1NaCl (0.25%), respectively. With rising NaCl concentration, MDA content, soluble protein (SP) content and the activities of enzymes like SOD, POD and CAT in germinating seeds were increased significantly, also, the contents of fPut, fSpd, fSpm, (fSpd+fSpm)/fPut, bPut, bSpd, and bSpm in the germinating tomato seeds all increased significantly, but (bSpd+bSpm)/bPut did not change much. Compared with the seeds in CK, the seeds treated with 50 mmol L-1 NaCl did not change much in conjugated Put (cPut), but increased significantly in the contents of cSpd, and cSpm and the ratio of (cSpd+cSpm)/cPut. With increasing NaCl concentration, the seeds declined gradually in cPut, cSpd, cSpm and (cSpd+cSpm)/cPut. It was also found that salt stress significantly reduced the content of total polyamines (TPAs) and free total polyamines (fPAs) in germinating tomato seeds, but increased the content of free total polyamines with rising NaCl concentration.[Conclusion] Salinity delays and even inhibits germination of tomato seeds, and has a much stronger inhibitory effect on establishment of the seedlings after germination. Tomato seeds per se have certain salt tolerance during their germination phase mainly due to the drastic increase in (fSpd+fSpm)/fPut, cSpd, cSpm, and significant accumulation of all the forms of bound polyamines, SS and SP in the seeds under salt stress, thus raising the seeds' antioxdation and osmotic adjustment capacities. However, when salt reaches as high as 150 mmol·L-1NaCl, the effects of salt stress raising cSpd and cSpm weaken, while the effects raising bound polyamine of all forms, fSpd and fSpm, and regulating SS and SP accumulation are still intensify.

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陈军,关欣,范翠枝,赵海燕,吴馨怡,韩鹰,顾志壮,郑青松,郑春芳.盐胁迫对番茄种子萌发中多胺形态变化和抗氧化的影响[J].土壤学报,2021,58(6):1598-1609. DOI:10.11766/trxb202002180051 CHEN Jun, GUAN Xin, FAN Cuizhi, ZHAO Haiyan, WU Xinyi, HAN Ying, GU Zhizhuang, ZHENG Qingsong, ZHENG Chunfang. Effects of Salt Stress on Form of Polyamine and Antioxidation in Germinating Tomato Seed[J]. Acta Pedologica Sinica,2021,58(6):1598-1609.

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  • 收稿日期:2020-02-18
  • 最后修改日期:2020-06-08
  • 录用日期:2020-08-18
  • 在线发布日期: 2020-12-10
  • 出版日期: 2021-11-11