首页 > 过刊浏览>2022年第59卷第2期 >536-544. DOI:10.11766/trxb202008310357
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生物质炭载体联合有益菌防控番茄土传青枯病的效果研究
作者:
  • 王孝芳

    王孝芳

    南京农业大学资源与环境科学学院/作物免疫重点实验室/江苏省固体有机废弃物资源化高新技术研究重点实验室/江苏省有机固体废弃物资源化协同创新中心/资源节约型肥料教育部工程研究中心/国家有机类肥料工程技术研究中心, 南京 210095
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  • 梅新兰

    梅新兰

    南京农业大学资源与环境科学学院/作物免疫重点实验室/江苏省固体有机废弃物资源化高新技术研究重点实验室/江苏省有机固体废弃物资源化协同创新中心/资源节约型肥料教育部工程研究中心/国家有机类肥料工程技术研究中心, 南京 210095
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  • 黄大鹏

    黄大鹏

    南京农业大学资源与环境科学学院/作物免疫重点实验室/江苏省固体有机废弃物资源化高新技术研究重点实验室/江苏省有机固体废弃物资源化协同创新中心/资源节约型肥料教育部工程研究中心/国家有机类肥料工程技术研究中心, 南京 210095
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  • 徐大兵

    徐大兵

    湖北省农业科学院植保土肥研究所, 武汉 430064
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  • 杨天杰

    杨天杰

    南京农业大学资源与环境科学学院/作物免疫重点实验室/江苏省固体有机废弃物资源化高新技术研究重点实验室/江苏省有机固体废弃物资源化协同创新中心/资源节约型肥料教育部工程研究中心/国家有机类肥料工程技术研究中心, 南京 210095
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  • 韦中

    韦中

    南京农业大学资源与环境科学学院/作物免疫重点实验室/江苏省固体有机废弃物资源化高新技术研究重点实验室/江苏省有机固体废弃物资源化协同创新中心/资源节约型肥料教育部工程研究中心/国家有机类肥料工程技术研究中心, 南京 210095
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  • 徐阳春

    徐阳春

    南京农业大学资源与环境科学学院/作物免疫重点实验室/江苏省固体有机废弃物资源化高新技术研究重点实验室/江苏省有机固体废弃物资源化协同创新中心/资源节约型肥料教育部工程研究中心/国家有机类肥料工程技术研究中心, 南京 210095
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  • 沈其荣

    沈其荣

    南京农业大学资源与环境科学学院/作物免疫重点实验室/江苏省固体有机废弃物资源化高新技术研究重点实验室/江苏省有机固体废弃物资源化协同创新中心/资源节约型肥料教育部工程研究中心/国家有机类肥料工程技术研究中心, 南京 210095
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基金项目:

国家重点研发计划项目（2018YFD0201401）、国家自然科学基金项目（42007025）和江苏省自然科学基金项目（SBK2020042856）资助


Effects of Probiotics with Biochar as Carrier Inhibiting Tomato Soil-borne Bacterial Wilt
Author:
  • WANG Xiaofang

    WANG Xiaofang

    College of Resources and Environmental Science Nanjing Agricultural University, Key Laboratory of Plant immunity, Jiangsu Provincial Key Lab of Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Waste Resource Utilization, Educational Ministry Engineering Center of Resource-saving fertilizers, National Engineering Research Center for Organic-based Fertilizers, Nanjing 210095, China
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  • MEI Xinlan

    MEI Xinlan

    College of Resources and Environmental Science Nanjing Agricultural University, Key Laboratory of Plant immunity, Jiangsu Provincial Key Lab of Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Waste Resource Utilization, Educational Ministry Engineering Center of Resource-saving fertilizers, National Engineering Research Center for Organic-based Fertilizers, Nanjing 210095, China
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  • HUANG Dapeng

    HUANG Dapeng

    College of Resources and Environmental Science Nanjing Agricultural University, Key Laboratory of Plant immunity, Jiangsu Provincial Key Lab of Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Waste Resource Utilization, Educational Ministry Engineering Center of Resource-saving fertilizers, National Engineering Research Center for Organic-based Fertilizers, Nanjing 210095, China
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  • XU Dabing

    XU Dabing

    Institute of Plant Protection and Soil Fertilizer, Hubei Academy of Agricultural Sciences, Wuhan 430064, China
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  • YANG Tinajie

    YANG Tinajie

    College of Resources and Environmental Science Nanjing Agricultural University, Key Laboratory of Plant immunity, Jiangsu Provincial Key Lab of Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Waste Resource Utilization, Educational Ministry Engineering Center of Resource-saving fertilizers, National Engineering Research Center for Organic-based Fertilizers, Nanjing 210095, China
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  • WEI Zhong

    WEI Zhong

    College of Resources and Environmental Science Nanjing Agricultural University, Key Laboratory of Plant immunity, Jiangsu Provincial Key Lab of Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Waste Resource Utilization, Educational Ministry Engineering Center of Resource-saving fertilizers, National Engineering Research Center for Organic-based Fertilizers, Nanjing 210095, China
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  • XU Yangchun

    XU Yangchun

    College of Resources and Environmental Science Nanjing Agricultural University, Key Laboratory of Plant immunity, Jiangsu Provincial Key Lab of Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Waste Resource Utilization, Educational Ministry Engineering Center of Resource-saving fertilizers, National Engineering Research Center for Organic-based Fertilizers, Nanjing 210095, China
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  • SHEN Qirong

    SHEN Qirong

    College of Resources and Environmental Science Nanjing Agricultural University, Key Laboratory of Plant immunity, Jiangsu Provincial Key Lab of Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Waste Resource Utilization, Educational Ministry Engineering Center of Resource-saving fertilizers, National Engineering Research Center for Organic-based Fertilizers, Nanjing 210095, China
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Fund Project:

Supported by the National Key R&D Program of China (No. 2018YFD0201401), the National Natural Science Foundation of China (No. 42007025) and the Natural Science Foundation of Jiangsu Province of China (No. SBK2020042856)

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    摘要:

    土传青枯病是由青枯菌（Ralstonia solanacearum）引起的一种细菌性病害。根际有益细菌在青枯病的防控中发挥着重要作用，其在根际有效定殖是发挥生防作用的前提。以玉米秸秆、木块（松木）和稻壳为原料制成的3种生物质炭为有益菌Bacillus amyloliquefaciens T-5的载体，探究生物质炭对有益菌防控番茄土传青枯病效果的影响，并利用室内模拟试验探究生物质炭对青枯菌的吸附、固持以及对根系分泌物的吸附作用，旨在阐述施用生物质炭提升有益菌T-5抑制病原青枯菌能力的可能机制。温室试验结果表明：单独施用3种生物质炭均显著降低青枯病的发病率和根际青枯菌的数量，其中具有高比表面积的木块生物质炭的防控效率达到60.56%。3种生物质炭作为有益菌T-5的载体均能够显著提升有益菌T-5的根际定殖数量及其防病效率，其中木块生物质炭的提升效果最好。与仅接种青枯菌的对照相比，木块生物质炭与有益菌T-5组合处理的根际青枯菌数量降幅达97.42%；与单独有益菌T-5处理相比，有益菌T-5以木块生物质炭为载体使其根际定殖数量提高了5.71倍。进一步研究发现，木块生物质炭能够有效吸附青枯菌，吸附量接近90.00%，且能够有效固持吸附的青枯菌，青枯菌的逃离降低了96.66%。此外，生物质炭不仅可以有效吸附根系分泌物，且有益菌T-5能够有效利用这些根系分泌物并抑制青枯菌的生长。生物质炭作为生防有益菌的载体，促进根际有益菌的定殖能力，并吸附、固持病原菌，有效抑制土传青枯病的发生。

    Abstract:

    [Objective] Tomato wilt is a kind of destructive soil-borne disease caused by Ralstonia solanacearum. In controlling the disease, rhizosphere probiotic play an important role. They reduce pathogen density and dull disease dynamics via resource competition and competitive exclusion. Their effective colonization in the rhizosphere is the precondition of their wilt controlling effect. As a porous material with adsorptive properties, biochar is used as a promising strategy to improve soil structure and fertility. Moreover, biochar has a great potential to inhibit soil-borne diseases, by transferring root exudates off rhizosphere and providing shelter for probiotics. To improve biocontrol efficiency of the probiotics, it is proposed that biochar is used as a carrier to improve their colonization in rhizosphere.[Method] In this study, wilt controlling effect of the application of biochar and probiotics in combination was evaluated and mechanisms underlying the potential were explored. To the end, a pot experiment was conducted to test three different kinds of biochar (derived from maize straw, pine wood and rice husk, separately) applied together with probiotic Bacillus amyloliquefaciens T-5 in controlling tomato bacterial wilt. And then an in-lab experiment was carried out too to measure efficiencies of the biochars adsorbing of pathogens and tomato root exudates in vitro. In the end, resource competition and direct toxin production of Bacillus amyloliquefaciens T-5 and their effects on pathogens were determined with simulated root exudates.[Result] Among the three kinds of biochar, the one derived from wood biochar is the highest in specific surface area (SSA) and absorption capacity, reaching up to 395.88 mm2 and 116.4 mg·g-1, respectively. The pot experiment showed that the application of biochar, regardless of kind, significantly reduced the incidence of bacterial wilt and pathogen density in rhizosphere. Wood biochar performed the best. When applied alone it decreased the incidence of bacterial wilt by 60.56%, and when applied in combination with probiotic Bacillus amyloliquefaciens T-5, it reduced pathogen density by about 97.42%, while increasing probiotic colonization by about 5.71 times. In exploring mechanisms of such potentials, it was found that 1) Biochar effectively adsorbed pathogen R. solanacearum, and wood biochar was the highest in adsorption capacity, reaching up to 90.00%, and fixing the adsorbed R. solanacearum by 94.66%; 2) Biochars absorbed root exudates, which were used by probiotic T-5 as carbon source for growth, thus inhibiting the growth of R. solanacearum.[Conclusion] As a carrier of probiotics, biochar, once applied together with probiotics can significantly decrease incidence of bacterial wilt by adsorbing root exudates probiotics relay as nutrient resources for growth and restricting the mobile ability of pathogen. The findings in this paper explain the mechanism of combined application of biochar and biocontrol bacteria controlling soil-borne tomato bacterial wilt and may serve as a theoretical basis for developing an environment-friendly, high efficiency and stable biocontrol strategy.

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王孝芳,梅新兰,黄大鹏,徐大兵,杨天杰,韦中,徐阳春,沈其荣.生物质炭载体联合有益菌防控番茄土传青枯病的效果研究[J].土壤学报,2022,59(2):536-544. DOI:10.11766/trxb202008310357 WANG Xiaofang, MEI Xinlan, HUANG Dapeng, XU Dabing, YANG Tinajie, WEI Zhong, XU Yangchun, SHEN Qirong. Effects of Probiotics with Biochar as Carrier Inhibiting Tomato Soil-borne Bacterial Wilt[J]. Acta Pedologica Sinica,2022,59(2):536-544.

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  • 收稿日期:2020-08-31
  • 最后修改日期:2020-12-21
  • 录用日期:2021-02-26
  • 在线发布日期: 2021-03-01
  • 出版日期: 2022-02-11
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