首页 > 过刊浏览>2021年第58卷第3期 >568-577. DOI:10.11766/trxb202008210474
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土壤宏病毒组的研究方法与进展
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中图分类号:

S154.3

基金项目:

国家重点研发计划项目（2018YFC1803100）、中国科协青年人才托举工程（2018QNRC001）、国家自然科学基金面上项目（41771350）资助


Review in the Soil Virus Metagenome Analytical Methods and Progress
Author:
Fund Project:

Supported by the National Key Research and Development Program of China（No.2018YFC1803100）、Young Elite Scientists Sponsorship Program by CAST（NO.2018QNRC001）and National Natural Science Foundation of China（No. 41771350）

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    摘要:

    土壤是病毒遗传多样性的储存库，但由于土壤自身特性及技术手段的限制，基于传统培养方法对土壤病毒的研究及功能认知存在局限性。宏病毒组学技术能直接从土壤环境样品中获取病毒基因组，随后通过高通量测序、拼接组装、ORF预测，最终可对病毒基因进行功能注释，极大地丰富了对土壤病毒功能的认识。本文在阐释土壤病毒DNA提取、测序与病毒判别、功能基因注释等研究方法的基础上，重点探讨了单株噬菌体基因组，及近年来国内外土壤与极端陆地环境中宏病毒组研究进展。并对土壤宏病毒基因组研究的前沿和发展趋势进行了总结，强调了土壤病毒研究的整体化、技术流程规范化以及病毒资源库完善化的重要性。

    Abstract:

    Soil is one of the most important reservoirs of virus genetic diversity. Due to the limitation of currently-available method for isolating and cultivating soil microorganisms, the overall diversity and function of the soil viruses remain largely unknown. Thanks to the development in the viral metagenome analysis, it is possible to directly obtain viromes from soil samples through high throughput sequencing, splicing assemble, ORF prediction, and protein annotation, which greatly enrich the understanding of soil viral functions. This review briefly summarizes the analytical methods that are extensively used in the soil virus metagenomic studies, including soil virus DNA extraction, sequencing and virus identification, functional gene annotation and etc. Meanwhile, the research progress in the phage genomes harbored in the culturable bacterial strains, and the viral metagenomes in terrestrial ecosystems were reviewed as well. This work highlights the significance of integrating currently-available virus analytical techniques, building standard viral analysis procedures, and optimizing the virus relevant databases.

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祁慧鹓,郑晓璇,孙明明,王金锋,马迎飞,朱冬,王风贺,蒋新,叶茂.土壤宏病毒组的研究方法与进展[J].土壤学报,2021,58(3):568-577. DOI:10.11766/trxb202008210474 QI Huiyuan, ZHENG Xiaoxuan, SUN Mingming, WANG Jinfeng, MA Yingfei, ZHU Dong, WANG Fenghe, JIANG Xin, YE Mao. Review in the Soil Virus Metagenome Analytical Methods and Progress[J]. Acta Pedologica Sinica,2021,58(3):568-577.

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  • 收稿日期:2020-08-21
  • 最后修改日期:2020-10-13
  • 录用日期:2020-11-12
  • 在线发布日期: 2020-12-07
  • 出版日期: 2021-05-11
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