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氮素水平对土壤甲烷氧化和硝化微生物相互作用的影响
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国家重点研发计划项目（2016YFD0200302）、国家自然科学基金项目（41907026）和中国博士后科学基金项目（2020T130387，2019M652448）资助


Effects of Nitrogen Levels on Interactions between Active Nitrifiers and Methanotrophs
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National Key R&D Program of China, Natural Science Foundation of China, China Postdoctoral Science Foundation

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    摘要:

    甲烷氧化微生物和氨氧化微生物均是既可以氧化甲烷（CH4）又可以氧化氨（NH3），氨氧化是硝化作用的限速步骤，也是好氧土壤氧化亚氮（N2O）排放的主要生物路径。选取内蒙古草原围封禁牧土壤为研究对象，利用稳定同位素核酸探针技术（DNA-SIP）探讨不同氮水平下土壤活性甲烷氧化微生物与硝化微生物及其相互作用机制。结果发现低氮添加促进甲烷氧化活性，而高氮添加抑制甲烷氧化活性；低氮和高氮添加均显著增强硝化活性。基于DNA-SIP的高通量测序结果发现Methylobacter MOB和Nitrosospira AOB/Nitrospira NOB分别是该土壤的主要活性甲烷氧化和硝化微生物。网络结构分析发现Methylobacter MOB和Nitrosospira AOB/Nitrospira NOB存在显著负相关关系，进一步证明活性甲烷氧化和硝化微生物之间存在竞争性相互作用。以上结果表明，氮素水平影响草原土壤甲烷氧化和硝化微生物的相互作用，研究结果为采取措施调控草原土壤CH4的汇和N2O的源功能以及减缓气候变暖的进程提供理论基础。

    Abstract:

    [Objective] Ammonia oxidation is the limited step of nitrification and it is the main microbial pathway of N2O emissions in aerobic soils. Grassland soils are sinks for CH4and sources of N2O. However, the mechanisms of the interactions between methane and ammonia oxidation under various N levels in grassland soils remain elusive.[Method] A microcosm experiment was implemented using Inner Mongolia grassland soils based on DNA-Stable-Isotope-Probing (DNA-SIP), and the methane and ammonia oxidation under various N levels was studied.[Result] The results showed that methane-oxidizing activity was stimulated by low N while it was suppressed by high N application. Contrarily, the nitrification activity was stimulated by both low and high N addition. The Methylobacter lineage of methane-oxidizing bacteria(MOB) were the active players in methane oxidation, while nitrifying communities dominated by Nitrosospira lineage of AOB and Nitrospira-like nitrite-oxidizing bacteria (NOB) were the active players in nitrification. Also, the network analysis unraveled significant negative relationships between active Methylobacter MOB and Nitrosospira AOB/Nitrospira NOB, which suggested competitive interactions between active nitrifiers and methanotrophs in grassland soils.[Conclusion] The interactions between active methanotrophs and nitrifiers can be affected by N levels. Our results provide theoretical support for regulating CH4 sinks and N2O sources of grassland soils and for greenhouse gas mitigation.

    参考文献
    [1] Stocker T F,Qin D,Plattner G K,et al. Climate Change 2013:The physical science basis. contribution of working group I to the fifth assessment report of IPCC the intergovernmental panel on climate change[EB/OL]. 2014
    [2] Shcherbak I,Millar N,Robertson G P. Global metaanalysis of the nonlinear response of soil nitrous oxide(N2O) emissions to fertilizer nitrogen[J]. Proceedings of the National Academy of Sciences of the United States of America,2014,111(25):9199-9204.
    [3] Kolb S. The quest for atmospheric methane oxidizers in forest soils[J]. Environmental Microbiology Reports,2009,1(5):336-346.
    [4] Stein L Y,Roy R,Dunfield P F. Aerobic methanotrophy and nitrification:Processes and connections[M]//Encyclopedia of life sciences. John Wiley & Sons Inc,2012
    [5] Jia Z J,Cai Z C. Methane consumption in relation to ammonia oxidation in paddy soils[J]. Rural Eco-Environment,2003,19(4):40-44.[贾仲君,蔡祖聪. 稻田甲烷氧化与铵氧化关系研究进展[J]. 农村生态环境,2003,19(4):40-44.]
    [6] Sala O E,Austin A T,Vivanco L. Temperate grassland and shrubland ecosystems[M]//Encyclopedia of Biodiversity. Amsterdam:Elsevier,2001:627-635.
    [7] Pan H,Xie K X,Zhang Q C,et al. Archaea and bacteria respectively dominate nitrification in lightly and heavily grazed soil in a grassland system[J]. Biology and Fertility of Soils,2018,54(1):41-54.
    [8] Mo Y L,Jin F,Zheng Y,et al. Succession of bacterial community and methanotrophy during lake shrinkage[J]. Journal of Soils and Sediments,2020,20(3):1545-1557.
    [9] Deng Y C,Che R X,Wang F,et al. Upland Soil Cluster Gamma dominates methanotrophic communities in upland grassland soils[J]. Science of the Total Environment,2019,670:826-836.
    [10] Cai Y F,Jia Z J. Research progress of atmospheric methane oxidizers in soil[J]. Acta Microbiologica Sinica,2014,54(8):841-853.[蔡元锋,贾仲君. 土壤大气甲烷氧化菌研究进展[J]. 微生物学报,2014,54(8):841-853.]
    [11] Daebeler A,Bodelier P L,Yan Z,et al. Interactions between Thaumarchaea,Nitrospira and methanotrophs modulate autotrophic nitrification in volcanic grassland soil[J]. The ISME Journal,2014,8(12):2397-2410.
    [12] Zheng Y,Huang R,Wang B Z,et al. Competitive interactions between methane-and ammonia-oxidizing bacteria modulate carbon and nitrogen cycling in paddy soil[J]. Biogeosciences,2014,11(12):3353-3368.
    [13] Jia Z J,Conrad R. Bacteria rather than Archaea dominate microbial ammonia oxidation in an agricultural soil[J]. Environmental Microbiology,2009,11(7):1658-1671.
    [14] Francis C A,Roberts K J,Beman J M,et al. Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean[J]. Proceedings of the National Academy of Sciences of the United States of America,2005,102(41):14683-14688.
    [15] Rotthauwe J H,Witzel K P,Liesack W. The ammonia monooxygenase structural gene amoA as a functional marker:Molecular fine-scale analysis of natural ammonia-oxidizing populations[J]. Applied and Environmental Microbiology,1997,63(12):4704-4712.
    [16] Kolb S,Knief C,Stubner S,et al. Quantitative detection of methanotrophs in soil by novel pmoA-targeted real-time PCR assays[J]. Applied and Environmental Microbiology,2003,69(5):2423-2429.
    [17] Stubner S. Enumeration of 16S rDNA of Desulfotomaculum lineage 1 in rice field soil by real-time PCR with SybrGreenTM detection[J]. Journal of Microbiological Methods,2002,50(2):155-164.
    [18] Quast C,Pruesse E,Yilmaz P,et al. The SILVA ribosomal RNA gene database project:Improved data processing and web-based tools[J]. Nucleic Acids Research,2013,41(D1):D590-D596.
    [19] Bodelier P L E,Hahn A P,Arth I R,et al. Effects of ammonium-based fertilisation on microbial processes involved in methane emission from soils planted with rice[J]. Biogeochemistry,2000,51(3):225-257.
    [20] Zhang L H,Yuan F H,Bai J H,et al. Phosphorus alleviation of nitrogen-suppressed methane sink in global grasslands[J]. Ecology Letters,2020,23(5):821-830.
    [21] Zhao J,Cai Y F,Jia Z J. The pH-based ecological coherence of active canonical methanotrophs in paddy soils[J]. Biogeosciences,2020,17(6):1451-1462.
    [22] Li Y,Liu Y W,Pan H,et al. Impact of grazing on shaping abundance and composition of active methanotrophs and methane oxidation activity in a grassland soil[J]. Biology and Fertility of Soils,2020,56(6):799-810.
    [23] Hao Q Q,Liu F H,Zhang Y C,et al. Methylobacter accounts for strong aerobic methane oxidation in the Yellow River Delta with characteristics of a methane sink during the dry season[J]. Science of the Total Environment,2020,704:135383.
    [24] Sultana N,Zhao J,Zheng Y,et al. Stable isotope probing of active methane oxidizers in rice field soils from cold regions[J]. Biology and Fertility of Soils,2019,55(3):243-250.
    [25] Huang X R,Zhao J,Su J,et al. Neutrophilic bacteria are responsible for autotrophic ammonia oxidation in an acidic forest soil[J]. Soil Biology & Biochemistry,2018,119:83-89.
    [26] Whitman WB,Rainey F,Kämpfer P,et al. Bergey's manual of systematics of Archaea and Bacteria[M]. Hoboken,New Jersey:Wiley,2015.
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潘红,李勇,孟春梅,郑燕,刘杏梅,诸葛玉平,贾仲君,邸洪杰,徐建明.氮素水平对土壤甲烷氧化和硝化微生物相互作用的影响[J].土壤学报,2022,59(2):557-567. DOI:10.11766/trxb202101050577 PAN Hong, LI Yong, MENG Chunmei, ZHENG Yan, LIU Xingmei, ZHUGE Yuping, JIA Zhongjun, DI Hongjie, XU Jianming. Effects of Nitrogen Levels on Interactions between Active Nitrifiers and Methanotrophs[J]. Acta Pedologica Sinica,2022,59(2):557-567.

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  • 收稿日期:2021-01-05
  • 最后修改日期:2021-03-24
  • 录用日期:2021-04-30
  • 在线发布日期: 2021-05-07
  • 出版日期: 2022-02-11
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