【Objective】 Low pH stress is one of the major factors limiting crop production on acidic soils. It often coexists with metal ion toxicity and this makes it difficult to explore directly the effect of pH in acidic soils. So far, the mechanisms of low pH stress in rice is poorly understood. 【Method】Two rice varieties with different low pH tolerance, Kasalath (low pH-tolerant) and Jinguoyin (low pH-sensitive), were selected to (i) study the relationship between low pH stress and the accumulation of nitric oxide (NO) and reactive oxygen species (ROS), and (ii) explore the regulatory relationship between NO and ROS under low pH stress. 【Result】Low pH caused the accumulation of NO and ROS in the root tips of Jinguoyin, but there was no significant change in Kasalath. The NO scavenger cPTIO reduced NO and ROS accumulation in root tips of Jinguoyin. Feedback inhibitor of nitrate reductase Gln significantly reduced NO content in the root tips of Jinguoyin under low pH, while L-NAME, a nitric oxide synthase inhibitor, did not affect NO content in the root tips of Jinguoyin. Low pH significantly increased the expression of nitrate reductase genes NIA1, NIA2 and NIA3 in Jinguoyin, and also increased the activity of nitrate reductase.【Conclusion】Low pH stress of Jinguoyin was related to the NO-mediated ROS accumulation. The NO signal generated under low pH stress is mainly synthesized by nitrate reductase through increasing the expression of NIA1 and NIA2.