水稻土厌氧氨氧化对不同耕作方式与秸秆还田的响应
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1.湖南省土壤肥料研究所;2.湖南农业大学资源环境学院

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Response of paddy soil anammox bacteria to rice straw returning and different tillage practices
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1.Hunan Soil and Fertilizer Institute;2.College of Environment and Resources, Hunan Agricultural University

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    摘要:

    【目的】研究稻田土壤厌氧氨氧化微生物活性、丰度和群落结构对不同耕作方式与秸秆还田的响应,为稻田土壤氮素管理提供科学依据。【方法】设置3个不同耕作方式(翻耕、旋耕、免耕)+秸秆还田处理,同时设置翻耕+秸秆不还田处理作为对照,采用15N示踪法、荧光定量PCR及Illumina测序分别分析厌氧氨氧化菌潜在活性、功能基因hzsB拷贝数及群落组成。【结果】秸秆还田条件下,厌氧氨氧化菌潜在活性表现为免耕>旋耕>翻耕(p<0.05),对照与旋耕+秸秆还田处理无显著性差异。各处理之间厌氧氨氧化菌功能基因(hzsB)拷贝数无显著性差异。Illumina测序从属水平鉴定出Kuenenia和Scalindua两种已知的厌氧氨氧化菌及部分未鉴定属,且各处理间二者的相对丰度具有负相关关系(p<0.05)。相关性分析结果表明,厌氧氨氧化菌活性与土壤反硝化活性显著相关,与功能基因拷贝数及群落组成无显著相关性。【结论】水稻田长期秸秆还田和不同耕作方式改变了厌氧氨氧化菌活性和群落组成,但其数量未发生显著变化。水稻田秸秆还田加翻耕可一定程度上抑制土壤氮损失,而秸秆还田与免耕结合的田间管理方式提高了土壤氮素损失潜力。

    Abstract:

    【Objective】This study was carried out to reveal the response of paddy soil anammox bacterial activity, abundance and community structure to rice straw returning and different tillage practices, which may provide scientific basis for nitrogen (N) management of paddy field. 【Method】Three different till treatments (conventional till, rotary till and no till)+rice straw returning were set up, respectively, with conventional till but no rice straw returning as control. The potential activity, functional gene (hzsB) abundance and community structure of anammox bacteria were analyzed by 15N tracing, fluorescent quantitative PCR and Illumina sequencing, respectively.【Result】Anammox activity showed significant difference and the values could be ranked as no till > rotary till > conventional till (p<0.05), while no significant difference was observed between control and rotary tillage+rice straw. No significant difference was detected in copy number of hzsB among treatments. Two known anammox bacteria (Kuenenia and Scalindua) and some unidentified genera were identified by Illumina sequencing, and the relative abundance of Kuenenia and Scalindua showed negative correlation (p<0.05). Pearson correlation analysis showed that anammox activity was significantly correlated with denitrification activity, but not with hzsB abundance and community composition. 【Conclusion】 We conclude that anammox activity and community composition, but no the functional gene abundance, respond to long term straw returning and different tillage practices. Rice straw returning+conventional till in rice field may inhibit N loss to some extent, while rice straw returning+no tillage increased soil N loss potentially.

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孙梅,聂三安,龙泽东,李超,王华,肖小平,罗尊长,孙耿.水稻土厌氧氨氧化对不同耕作方式与秸秆还田的响应[J].土壤学报,,[待发表]
Sun Mei, Nie San An, Long Ze Dong, Li Chao, Wang Hua, Xiao Xiao Ping, Luo Zun Chang, sungeng. Response of paddy soil anammox bacteria to rice straw returning and different tillage practices[J]. Acta Pedologica Sinica,,[In Press]

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