首页 > 过刊浏览>2022年第59卷第6期 >1695-1703. DOI:10.11766/trxb202109010267
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水稻土厌氧氨氧化对不同耕作方式与秸秆还田的响应
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S154.2

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区域创新发展联合基金(U19A2048)、国家重点研发计划项目(2016YFD0300901-03)和国家自然科学基金项目(42177288)共同资助


Response of Paddy Soil Anammox Bacteria to Rice Straw Returning and Different Tillage Practices
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Supported by the Joint Regional Innovation and Development Fund (No. U19A2048), the National Key Research and Development Program of China (No. 2016YFD0300901-03) , and the National Natural Science Foundation of China (No. 42177288)

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    摘要:

    研究稻田土壤厌氧氨氧化微生物活性、丰度和群落结构对不同耕作方式与秸秆还田的响应，可为稻田土壤氮素管理提供科学依据。设3个不同耕作方式（翻耕、旋耕、免耕）+秸秆还田处理，以翻耕+秸秆不还田处理作为对照，采用15N示踪法、荧光定量PCR及Illumina测序分析厌氧氨氧化菌潜在活性、功能基因hzsB拷贝数及群落组成。结果表明，秸秆还田显著改变了土壤理化性质，与不还田相比，土壤有机质、全氮、容重均呈明显增加趋势，尤其是免耕还田条件下差异显著。秸秆还田条件下，厌氧氨氧化菌潜在活性表现为免耕高于旋耕高于翻耕（P < 0.05），对照与旋耕+秸秆还田处理无显著性差异。各处理之间厌氧氨氧化菌功能基因（hzsB）拷贝数无显著性差异。Illumina测序从属水平鉴定出Kuenenia和Scalindua两种已知的厌氧氨氧化菌及部分未鉴定属，且各处理间二者的相对丰度具呈负相关（P < 0.05）。相关性分析结果显示，厌氧氨氧化菌活性与土壤反硝化活性显著相关，与功能基因拷贝数及群落组成无显著相关性。试验表明，免耕条件下秸秆还田显著提升了土壤容重，降低了土壤氧化势，可能是其厌氧氨化活性强的重要原因。与无机氮含量和土壤全氮等理化性质相比，水稻土氧化还原状况可能更好解释水稻土厌氧氨氧化活性，长期秸秆还田和不同耕作方式改变了厌氧氨氧化菌活性和群落组成，但其数量未发生显著变化。水稻田秸秆还田加翻耕可在一定程度提高土壤透气性，降低厌氧氨氧化活性，而秸秆还田与免耕结合则可能降低土壤通透性并导致更多的土壤氮素损失。

    Abstract:

    Objective This study was carried out to evaluate the response of paddy soil anammox bacterial activity, abundance and community structure to rice straw returning and different tillage practices.Method Three different till treatments (conventional till, rotary till and no-till) + rice straw returning were set up, with conventional till and no rice straw returning as control. The potential activity, functional gene (hzsB) abundance and community structure of anammox bacteria were analyzed by 15N tracing, fluorescent quantitative PCR and Illumina sequencing, respectively.Result Anammox activity showed a significant difference and the values ranked as no-till > rotary till > conventional till(P < 0.05), while no significant difference was observed between control and rotary tillage+rice straw. Also, no significant difference was detected in the copy number of hzsB among treatments. Two known anammox bacteria (Kuenenia and Scalindua) and some unidentified genera were identified by Illumina sequencing, and the relative abundance of Kuenenia and Scalindua showed a negative correlation (P < 0.05). Pearson correlation analysis showed that anammox activity was significantly correlated with denitrification activity, but not with hzsB abundance and community composition.Conclusion We conclude that anammox activity and community composition but not the functional gene abundance respond to long term straw returning and different tillage practices. Rice straw returning + conventional till in rice field may inhibit N loss to some extent, while rice straw returning + no-tillage increased soil N loss. This study may provide a scientific basis for nitrogen (N) management in paddy fields.

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孙梅,聂三安,龙泽东,李超,王华,肖小平,罗尊长,孙耿.水稻土厌氧氨氧化对不同耕作方式与秸秆还田的响应[J].土壤学报,2022,59(6):1695-1703. DOI:10.11766/trxb202109010267 SUN Mei, NIE San'an, LONG Zedong, LI Chao, WANG Hua, XIAO Xiaoping, LUO Zunchang, SUN Geng. Response of Paddy Soil Anammox Bacteria to Rice Straw Returning and Different Tillage Practices[J]. Acta Pedologica Sinica,2022,59(6):1695-1703.

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  • 收稿日期:2021-09-01
  • 最后修改日期:2021-11-11
  • 录用日期:2022-01-26
  • 在线发布日期: 2022-02-21
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