Supported by the National Natural Science Foundation of China (Nos. 32071642 and 41771298), the Natural Science Foundation of Jiangsu Province, China (No. BK20191511)
土传病害的绿色防控是当前农业、生态和环境领域中为建立资源节约型、环境友好型病虫害可持续治理技术体系的重要组成部分。将来自藓类泥炭和风化煤的腐殖酸添加至两个花生种植年限的土壤中(1年和6年),进行室内恒温培养。对土壤真菌进行内部转录间隔区(internal transcribed spacer,ITS)测序,运用非参数多因素方差分析(PerMANOVA)、相关性分析等方法阐释腐殖酸对土壤真菌群落的影响。结果表明:腐殖酸的添加显著影响了培养94 d以后的土壤真菌群落结构,而添加量对4个培养时间点的土壤真菌群落结构均可产生显著影响;同时腐殖酸显著改变了部分真菌属的相对丰度。腐殖酸对土壤真菌功能的影响,主要表现为显著改变了土壤中真菌功能营养型的组成。其中,随着腐殖酸添加量的增加,土壤植物病原菌的相对丰度均显著降低;相关性分析表明腐殖酸添加量与致病菌镰刀属(Fusarium spp.)和丝核菌属(Rhizoctonia spp.)的相对丰度呈显著负相关,而与拮抗菌青霉属(Penicilliumspp.)的相对丰度呈显著正相关。本研究表明,特定腐殖酸在适当添加量下可显著改变花生地的土壤真菌群落结构和功能营养型组成,尤其是降低了土壤中植物病原菌的相对丰度,但影响程度因花生种植年限的长短而异。本研究结果为腐殖酸生态功能应用拓展提供了理论依据,为土传病害绿色防控技术的研发提供新的视角。
【Objective】The negative impact of soil-borne diseases on peanut is enhanced for continuous planting. Thus, green prevention and control practices on soil-borne diseases are always an important part when establishing a resource-saving and environment-friendly sustainable control technology system. As a natural stimulant, humic acid has been shown to suppress the activity of soil-borne pathogenic fungi, and the inhibitory effect has a wide range and diverse manifestations. However, valid information is still lacking about the effects of humic acid on the microbial community. The objective of this work was to investigate the response of soil fungal communities under continuous peanut planting to humic acid from different origins, and to provide a theoretical basis for researching and developing a green-effective prevention and control method on soil-borne diseases in the continuous planting soil.【Method】 Different concentrations of humic acids from mossy peat and weathered coal were respectively added to two peanut planting soils (including 1 year and 6 years continuous planting), and soils were cultured with indoor thermostatic incubation. The soil fungi community was sequenced by Illumina MiSeq PE300. PerMANOVA, correlation analysis, and other methods were used to explain the effect of humic acid on the soil fungi community. 【Result】The results showed that the addition of humic acid had a significant effect on the fungal community structure of six years planting soil, while it had no significant effect on one-year planting soil. The concentration of humic acid both had a significant effect on the fungi community structure of the two planting soils. Effects of humic acid on soil fungi community structure at each culture time were as follows: humic acids significantly changed the fungi community structures of one-year planting soil at 94 d and 193 d, and of six years planting soil at 193 d. Also, the concentration of humic acids had significant effects on the fungi community structure of the two planting soils during the whole culture process. Humic acid also significantly changed the relative abundance of some fungi at the genus level. The effect of humic acid on soil fungal function can be mainly attributed to its significant alteration of the compositions of fungal functional trophic types in the two planting soils. It was also observed that the relative abundance of plant pathogens in soil decreased significantly with an increase in the concentration of humic acid. Spearman correlation analysis further detected that the relative abundances of Fusarium spp. and Rhizoctonia spp. were significantly negatively correlated with the concentration of humic acid, while the relative abundance of Penicillium spp. was significantly positively correlated. The correlation coefficients were -0.270, -0.138, and 0.172, respectively. 【Conclusion】The appropriate concentration of specific humic acid could significantly change the soil fungi community structure and functional trophic composition in peanut soils, especially in reducing the relative abundance of plant pathogens. Results of this study could provide a direct theoretical basis for expanding the application of the ecological function of humic acid, and provide a new perspective for researching and developing efficient green prevention and control technology of soil-borne diseases.
魏世平,吴萌,李朋发,刘佳,李桂龙,刘凯,刘明,李忠佩.腐殖酸对花生连作地土壤真菌群落结构的影响[J].土壤学报,2023,60(3):846-856. DOI:10.11766/trxb202111050447 WEI Shiping, WU Meng, LI Pengfa, LIU Jia, LI Guilong, LIU Kai, LIU Ming, LI Zhongpei. Effects of Humic Acid on Fungal Community Structure in a Peanut-continuous Cropping Soil[J]. Acta Pedologica Sinica,2023,60(3):846-856.复制