氮肥配施秸秆对土壤原生生物群落的影响*
DOI:
作者:
作者单位:

1.农业农村部环境保护科研监测所农业农村部产地环境污染防控重点实验室;2.喀喇沁旗农牧局;3.农业农村部环境保护科研监测所天津市农业环境与农产品安全重点实验室

作者简介:

通讯作者:

中图分类号:

X172

基金项目:


Effects of nitrogen fertilization combined with straw incorporation on soil protist community
Author:
Affiliation:

1.Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Key Laboratory of Original Agro-environment Pollution Prevention and Control of Ministry of Agriculture and Rural Affairs;2.Agricultural and Pastoral Bureau of Karakqin Banner;3.Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin Key Laboratory of Agro-environment and Agro-product Safety

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
    摘要:

    【背景】原生生物是土壤食物网的重要组成部分,在土壤养分循环中发挥重要作用,其群落组成变化在一定程度上能反映农业管理措施对土壤生态系统的影响。【目的】为研究氮肥配施秸秆对华北麦玉轮作田土壤原生生物群落的影响。【方法】本研究通过田间定位施肥试验,采用Illumina高通量测序技术对8种施肥处理下土壤原生生物群落的多样性、组成和结构差异进行分析,并结合土壤理化性质,探究驱动土壤原生生物群落变化的关键环境因子。【结果】结果表明,秸秆添加提高了土壤pH值,并增加了捕食型原生生物的相对丰度;各处理中,绿藻门(Chlorophyta)、锥足亚门(Conosa)、丝足虫门(Cercozoa)和纤毛虫门(Ciliophora)为优势类群,其中绿藻门(Chlorophyta)的相对丰度随氮肥施用浓度的增加而降低;在单施氮肥组施氮量300 kg/hm2(N3)处理中,与其他处理相比,顶复门(Apicomplexa)的相对丰度显著增加。隶属于丝足虫门(Cercozoa)、纤毛虫门(Ciliophora)等原生生物种群以捕食型为主,其比例在秸秆还田组随配施氮肥量的增加而升高。【结论】施肥与秸秆还田通过改变土壤理化性质,直接或间接影响土壤微生物群落,进而影响原生生物群落;相比单施氮肥组,秸秆还田使捕食型原生生物比例增加。本研究为探寻秸秆还田方式下氮肥适宜用量提供了理论依据和技术支撑,为华北地区农田生态系统健康评价和农业可持续发展提供参考依据。

    Abstract:

    【Background】 As an important part of soil food web, protists play an important role in soil nutrient cycling, and their community composition change can reflect the impact of agricultural management measures on soil ecosystem to a certain extent.【Objective】 To investigate the effects of nitrogen fertilizer combined with straw application on soil protozoa community in Wheat-corn rotation field of North China.【Methods】 In this study, Illumina high-throughput sequencing technology was used to analyze the diversity, composition and structure of soil protist communities under 8 fertilization treatments. The key environmental factors driving the change of soil protist communities were investigated by combining with the determination of soil physical and chemical properties.【Result】 The results showed that straw addition increased soil pH value and the relative abundance of predation protist. In all the treatments, Chlorophyta, Conosa, Cercozoa and Ciliophora were the dominant species, and the relative abundance of Chlorophyta decreased with the increase of nitrogen application concentration. Compared with other treatments, the relative abundance of Apicomplexa was significantly increased in the 300 kg/hm2 (N3) treatments. The protist populations belonging to Cercozoa, Ciliophora and other phyla were mainly predation species, and their proportion increased with the increase of nitrogen application rate in straw returning group.【Conclusion】 Fertilization and straw returning changed soil physical and chemical properties, directly or indirectly affected soil microbial community, and then affected the community of protists. Straw returning increased the Phagotrophs compared with N fertilizer group. This study provided theoretical basis and technical support for the exploration of suitable nitrogen fertilizer dosage under straw returning, and provided reference basis for farmland ecosystem health evaluation and agricultural sustainable development in North China.

    参考文献
    相似文献
    引证文献
引用本文

胡 菏,李胜君,王 蕊,吴 宪,李玉洁,赵建宁,李 刚,修伟明.氮肥配施秸秆对土壤原生生物群落的影响*[J].土壤学报,,[待发表]
Huhe, Lishengjun, Wangrui, Wuxian, liyujie, zhaojianning, Ligang, Xiuweiming. Effects of nitrogen fertilization combined with straw incorporation on soil protist community[J]. Acta Pedologica Sinica,,[In Press]

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数: