State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences
广泛分布在水-土界面之间的周丛生物是稻田生态系统物质迁移和能量交换的关键热区和必经之地，但至今鲜有研究关注稻田周丛生物的群落特征及其调控功能。为进一步了解周丛生物磷捕获功能，本研究选取我国主要稻区周丛生物，覆盖华南地区、长江中下游地区、东北地区三个水稻种植区，利用高通量测序技术分析周丛生物群落组成结构，结合PLS-PM和回归分析揭示影响稻田周丛生物捕获磷的因素。结果表明：不同地区稻田土壤理化性质存在显著差异（P < 0.05）。我国主要稻区周丛生物磷捕获能力由北到南、由西向东呈现递增的趋势。不同地区稻田周丛生物群落组成差异显著（P < 0.05）。在属水平上，华南地区周丛生物以Acinetobacter、Proteiniclasticum等原核微生物及Gregarina、Adriamonas等真核微生物为优势物种；长江中下游地区周丛生物以Bacillus、Cloacibacterium等原核微生物及Gregarina、Vermamoeba等真核微生物为主组成；在东北地区，周丛生物由Flavisolibacter、Anaerolinea为优势的原核微生物和以Adriamonas、Vermamoeba为优势的真核微生物组成。发现土壤理化性质（特别是土壤有机碳含量和田面水pH）与周丛生物群落多样性存在相关性。华南地区周丛生物中的微藻对于磷的捕获起到一定的促进作用，而东北地区周丛生物中的聚磷菌对磷的捕获有正效应。本研究通过对全国不同地区稻田周丛生物磷捕获能力的分析，丰富了对稻田周丛生物群落组成、结构及其调控磷循环的认识，也为将来开发生物技术回收利用稻田盈余磷提供了理论依据。
【Objective】The periphytic biofilms widely distributed between the water-soil interface are key hot areas and necessary places for material migration and energy exchange in the paddy ecosystem. However, few studies have paid attention to the community characteristics and regulatory functions of the paddy periphytic biofilm. 【Method】In order to further understand the function of periphytic biofilm biological phosphorus capture, this study selects the periphytic biofilms in the main rice areas in China, covering three rice planting areas in the South China, the middle and lower reaches of the Yangtze River, and the Northeast. The composition and structure of periphytic biofilms communities are analyzed using high-throughput sequencing technology, combined with PLS-PM and regression analysis to reveal factors affecting the capture of phosphorus by periphytic biofilms around the paddy field. 【Result】There are significant differences in soil physical and chemical properties in different paddy regions (P < 0.05). The phosphorus capture capacity of the periphytic biofilms in main rice regions in China showed an increasing trend from north to south and west to east. There are significant differences in the composition of the periphytic biofilms community of paddy fields in different regions (P < 0.05). At the genus level, the periphytic biofilms in South China are dominated by prokaryotic microorganisms such as Acinetobacter and Proteiniclasticum and eukaryotic microorganisms such as Gregarina and Adriamonas. The periphytic biofilms in the middle and lower reaches of the Yangtze River are mainly prokaryotic microorganisms such as Bacillus and Cloacibacterium and eukaryotic microorganisms such as Gregarina and Vermamoeba. In the Northeast, periphytic biofilms are composed of prokaryotic microorganisms dominated by Flavisolibacter and Anaerolinea and eukaryotic microorganisms dominated by Adriamonas and Vermamoeba. It was found that the physical and chemical properties of soil (especially soil organic carbon and pH of floodwater) correlated with the diversity of the periphytic biofilms community. The microalgae in the periphytic biofilms in South China play a certain role in promoting the capture of phosphorus, while the phosphorus accumulating bacteria in the periphytic biofilms in the northeast have a positive effect on the capture of phosphorus. 【Conclusion】This study provides deep insight into the understanding of the composition and structure of the periphytic biofilms and their regulation in the phosphorus cycle in paddy fields through analysis of the phosphorus capture capacity of periphytic biofilms in paddy fields in different regions of China. Also, this study provides a theoretical basis for the future development of biotechnology to recycle surplus phosphorous in paddy fields.
LU Wen-yuan, SUN Peng-fei, XU Ying, LIU Jun-zhuo, WU Yong-hong. Composition Structure and Phosphorus Entrapment Capacities of Periphytic Biofilms in Paddy Fields in Different Regions in Eastern China[J]. Acta Pedologica Sinica, DOI:10.11766/trxb202203220002,[In Press]