不同载体材料固定Sphingobium sp. PHE3对溶液中菲的降解研究
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作者单位:

1.中国科学院南京土壤研究所土壤环境与污染修复重点实验室;2.江苏省农业科学院农业资源与环境研究所

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国家重点研发计划项目(2020YFC1807000,2019YFC1804400,2019YFC1804203)、国家自然科学基金项目(42007133,41977137,21906162)和江苏省自然科学基金(BK20191107)资助


Effects of Different Carrier Materials on the Degradation of Phenanthrene by Sphingobium sp. PHE3
Author:
Affiliation:

1.Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences;2.Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences

Fund Project:

Supported by the National Key Research and Development Program of China(Nos. 2020YFC1807000,2019YFC1804400,2019YFC1804203),the National Natural Science Foundation of China(Nos. 42007133,41977137,21906162),and the Natural Science Foundation of Jiangsu Province(No. BK20191107)

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    摘要:

    以蒙脱石、针铁矿、玉米秸秆炭为载体,采用吸附挂膜法对菲降解菌株(革兰氏阴性菌鞘脂菌PHE3)进行固定化,并应用固定化微生物降解溶液中的菲。根据扫描电镜结果,该菌株在玉米秸秆炭载体表面附着生长数量最多,形态最优。添加蒙脱石和玉米秸秆炭显著(P<0.05)提高了微生物对菲的降解率,加快了菲的微生物降解速率,同时促进了细菌胞外聚合物(EPS)中多糖和蛋白质产量的增加,但添加针铁矿对菲的微生物降解和EPS的产生促进作用较弱。此外,鞘脂菌PHE3在形成生物膜的过程中可能有群体感应现象发生,能够分泌信号分子C8-HSL和C12-HSL,可能在一定程度上调控EPS的生成和污染物的降解。

    Abstract:

    The biodegradation of phenanthrene was investigated in the liquid phase by gram-negative bacteria Sphingobium sp. PHE3 immobilized on montmorillonite, goethite and maize straw-derived biochar. The results of scanning electron microscopy showed that there was the largest number of bacteria adhered to the surface of the biochar with the best morphology. The addition of montmorillonite and biochar significantly (P<0.05) improved the degradation rate of phenanthrene by microorganisms, as well as the increase of polysaccharide and protein production in bacterial extracellular polymeric substance (EPS). Nevertheless, there was no obvious effect on phenanthrene biodegradation and EPS production with the addition of goethite. Furthermore, quorum sensing might play a role in biofilm formation. The signal molecules C8-HSL and C12-HSL secreted by PHE3 might mediate the production of EPS and phenanthrene biodegradation to some extent.

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尹媛,生弘杰,王紫泉,相雷雷,付玉豪,卞永荣,蒋新,王芳.不同载体材料固定Sphingobium sp. PHE3对溶液中菲的降解研究[J].土壤学报,DOI:10.11766/trxb202202220071,[待发表]
Yin Yuan, Sheng Hongjie, Wang Ziquan, Xiang Leilei, Fu Yuhao, Bian Yongrong, Jiang Xin, Wang Fang. Effects of Different Carrier Materials on the Degradation of Phenanthrene by Sphingobium sp. PHE3[J]. Acta Pedologica Sinica, DOI:10.11766/trxb202202220071,[In Press]

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  • 收稿日期:2022-02-22
  • 最后修改日期:2022-04-20
  • 录用日期:2022-06-23
  • 在线发布日期: 2022-07-12
  • 出版日期: