【Objective】The objective of this study was to clarify the status of soil nutrient accumulation in the peach orchard of Taihu Watershed. It aimed to provide a guide for the green production mode of peach orchard. 【Method】In this study, a total of 66 soil samples were collected from 33 juicy peach orchards, with different cultivation years in the four main planting towns. Combined with field research and laboratory analysis, the fertilizer input status, soil nutrient content and ecological stoichiometry characteristics were analyzed. 【Result】The results showed that the average total nutrient inputs was N 527 kg·hm^-2, P₂O₅ 362 kg·hm^-2 and K₂O 545 kg·hm^-2, and the proportion of organic inputs were 27.8%, 15.4% and 15.6% respectively. Here chemical fertilizer was mainly NPK compound fertilizer, while the types of organic fertilizer applied mainly included rapeseed cake, livestock manure, and composting straw. Over-application of fertilizer could aggravate soil acidification and nutrient accumulation, soil pH in 0-20 cm and 20-40 cm soil layers of planted >10 years peach orchard both decreased by more than 1.5 units, which belonged to strongly acidic soil (4.5<pH<5.5). According to the 2^nd National Soil Survey of soil fertility classification standards, soil organic matter, total nitrogen, phosphorus and potassium in 0-20 cm and 20-40 cm soil layers reached level 3 or above, and the C: N, C: P and N: P ratios in 0-20 cm soil layer decreased with an increase of cultivation years. Soil alkali-hydrolytic nitrogen, available phosphorus and potassium of planted >10 years peach orchard reached level 1. The soil NO₃^--N concentration of the peach orchard was up to 19.3-33.1 mg·kg^-1. The annual apparent surplus of N, P₂O₅, K₂O were 343 kg·hm^-2, 238 kg·hm^-2 and 240 kg·hm^-1, respectively. 【Conclusion】The excessive nutrient input in the peach orchard has aggravated soil acidification and nutrient accumulation. A high concentration of nitrogen and phosphorus accumulated in the soil might increase the environmental loss risk. Therefore, it is necessary to put forward a green production model for the peach production.