首页 > 过刊浏览>2023年第60卷第5期 >1507-1519. DOI:10.11766/trxb202203170117
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植被恢复对黄土高原露天矿区土壤碳氮磷功能微生物类群的影响
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Q938.1+1

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国家自然科学基金项目（51974313， 41907405）资助


Effects of Vegetation Restoration on Functional Groups Related to Soil Carbon, Nitrogen and Phosphorus Cycles in Open-pit Mining Area of the Loess Plateau
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Supported by the National Natural Science Foundation of China (Nos. 51974313 and 41907405)

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    摘要:

    黄土高原矿区生态脆弱，植被恢复与土壤微生物介导的养分循环密切相关。厘清植被恢复对土壤碳氮磷功能微生物类群的影响及调控作用，对重建矿区生态恢复力及自维持机制至关重要。采用高通量qPCR芯片技术、随机森林模型和结构方程模型揭示黄土高原安太堡露天矿复垦排土场柠条（灌丛，BL）、油松（针叶林，CF）、刺槐（阔叶林，BF）、油松+榆树（混交林，MF）等4种植被恢复模式及毛白杨林（CK）对土壤理化性质、酶活性、碳氮磷功能微生物类群的影响及互馈机制。结果表明：BL、BF和MF对土壤养分积累优于CF，不同恢复处理过氧化氢酶（CAT）活性均显著高于CK，但β-葡萄糖苷酶（BG）活性和亮氨酸氨基肽酶（LAP）活性却显著下降；植被恢复显著改变碳氮磷相关功能微生物类群丰度，但变化趋势几乎一致；碳氮磷相关功能微生物类群与土壤硝态氮（NO3--N）呈显著正相关，与铵态氮（NH4+-N）呈显著负相关，参与碳循环、硝化过程和有机磷矿化的功能微生物类群与有效磷（AP）呈显著正相关；植被恢复通过直接影响CAT和AP来调控碳氮磷循环，或间接影响AP并与NO3--N、NH4+-N等共同调节碳氮磷功能微生物类群的丰度变化。本研究深化了对植被-土壤恢复的微生物学机制的认识，可助力黄土高原受损矿山生态修复。

    Abstract:

    【Objective】The ecology of mining areas in the Loess Plateau is relatively fragile, while the vegetation restoration is closely related to soil microbe-mediated nutrient cycling. This study was designed to clarify the influence and regulatory roles of vegetation restoration on the functional groups related to soil carbon, nitrogen and phosphorus cycles, which are significantly important to rebuilding the ecological resilience and self-sustaining mechanism of mining areas.【Method】In this study, high-throughput qPCR chip technology, random forest model and structural equation model were used to reveal the changes and mutual feeding mechanism of soil characteristics, enzyme activities, carbon, nitrogen and phosphorus functional groups under different vegetation restoration modes of Antaibao open-pit reclamation waste dump on the Loess Plateau. 【Result】 The results showed that Caragana korshinskii (bushwood, BL), Pinus tabuliformis (coniferous forest, CF), Robinia pseudoacacia (broad-leaved forest, BF) and Robinia pseudoacacia + Ulmus pumila (mixed forest, MF) were superior to soil nutrient accumulation compared with Populus simonii forest (CK). Compared to CF, soil organic carbon (SOC) of BL, BF and MF increased by 82.26%, 168% and 56.65%, respectively. The total nitrogen (TN) of BL, BF and MF enhanced by 68.31%, 130% and 46.75% comparing with CF, while the available phosphorus (AP) contents increased by 10.41%, 27.65% and 20.89%, respectively. Nevertheless, these were still significantly lower than that of CK (P<0.05). The soil catalase (CAT) activities of BL, CF, BF and MF increased by 95.6%, 101.0%, 46.4% and 120.0% respectively, and are significantly higher than that of CK (P<0.05). Also, the soil β-glucosidase (BG) and leucine aminopeptidase (LAP) activities decreased significantly (P<0.05). The β-glucosidase activities of BL, CF, BF and MF decreased by 66.5%, 67.0%, 58.9% and 59.8%, while the leucine aminopeptidase activities decreased by 57.8%, 67.7%, 78.0% and 67.7%, respectively. The different modes of vegetation restoration significantly changed the relative abundances of functional groups related to carbon, nitrogen and phosphorus (P<0.05), whereas the variation tendencies always keep a consistent correspondence. The functional groups related to carbon, nitrogen and phosphorus were significantly positively correlated with soil nitrate nitrogen (P<0.001), but they presented negative correlations with ammonium nitrogen (P<0.01). The functional groups involved in the carbon cycle, nitrification process and organic phosphorus mineralization were significantly positively correlated with available phosphorus (P<0.01). In addition, results of the structural equation model showed that vegetation restoration could directly affect CAT and AP, thereby regulating the carbon, nitrogen and phosphorus cycle. Otherwise, the vegetation restoration could affect soil AP indirectly, thereupon then regulating the abundances of carbon, nitrogen and phosphorus functional groups, together with other soil characteristics, such as nitrate nitrogen or ammonium nitrogen. 【Conclusion】 This study might help deepen the knowledge about soil microbiology mechanism of vegetation restoration, which will furtherly help the ecological restoration of damaged mines in the Loess Plateau.

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陈浮,赵姣,马静,张琦,朱燕峰,骆占斌.植被恢复对黄土高原露天矿区土壤碳氮磷功能微生物类群的影响[J].土壤学报,2023,60(5):1507-1519. DOI:10.11766/trxb202203170117 CHEN Fu, ZHAO Jiao, MA Jing, ZHANG Qi, ZHU Yanfeng, LUO Zhanbin. Effects of Vegetation Restoration on Functional Groups Related to Soil Carbon, Nitrogen and Phosphorus Cycles in Open-pit Mining Area of the Loess Plateau[J]. Acta Pedologica Sinica,2023,60(5):1507-1519.

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  • 收稿日期:2022-03-17
  • 最后修改日期:2022-06-15
  • 录用日期:2022-07-25
  • 在线发布日期: 2022-07-29
  • 出版日期: 2023-09-28
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