Nanjing Normal University
National Natural Science Foundation of China (Nos. 42090065, 42077031), Yunnan Province Expert Workstation Project (No. 202205AF150039), and the Reserve Talent Project of Young and Middle-aged Academic and Technical Leaders of Yunnan Province (No. 202005AC160043)
为研究强还原土壤处理(Reductive soil disinfestation, RSD)对连作土壤微生物群落稳定性的影响,以及微生物群落稳定性变化与其功能的联系,以云南省石屏县连续种植多年的洋桔梗栽培土壤为研究对象,设置4个处理：对照(CK)；分别添加高碳氮比有机物料(C/N 122, 15 t.hm-2, SB),低碳氮比有机物料(C/N 19, 15 t.hm-2, BD),高、低碳氮比有机物料等质量混合(15 t.hm-2, SB+BD)的RSD处理。采用Biolog微平板法、定量PCR及高通量测序等技术手段分析了土壤微生物群落稳定性及碳、氮代谢功能。结果表明,与CK相比,RSD处理能够大幅降低细菌群落组成和丰度的稳定性,且SB+BD处理的影响较SB和BD处理强烈,但对真菌群落组成和丰度的稳定性无显著影响。同时,RSD处理能够显著提高细菌和真菌群落相互作用关系的稳定性,且SB和BD处理对其提升效果优于SB+BD处理。回归分析表明,土壤微生物群落的相互作用关系稳定性与其组分和丰度的稳定性关系密切。相关性分析显示,土壤微生物群落稳定性与其活性、碳代谢功能以及反硝化能力高度相关。综上,强还原土壤处理能够通过降低微生物群落组成和丰度的稳定性来提高类群间相互作用关系的稳定性,促进土壤微生物活性恢复和群落生态功能改善。
【Objective】Reductive soil disinfestation (RSD) is an effective agriculture practice to conquer continuous cropping obstacles by the elimination of soil-borne pathogens, degradation of allelochemicals, improvement of soil microbial community structure, and restoration of soil microbial functions. However, the effects of RSD practice on the stability of the mono-cropped soil microbial community are still unknown. Moreover, the relationship between soil microbial community stability and functions also needs to be studied. Therefore, this study was oriented to explore the relationship between the stability and function of soil microbial communities driven by reductive soil disinfestation. 【Method】A field experiment, designed to have four treatments, i.e. CK (control without soil treatment); SB (RSD incorporated with 15 t·hm-2 organic substrate with C/N ratio of 122); BD (RSD incorporated with 15 t·hm-2 organic substrate with C/N ratio of 19); and SB+BD (RSD incorporated with 15 t·hm-2 organic substrates containing both high and low C/N ratios with equal mass), was carried out in a lisianthus mono-cropped soils in Shiping County, Yunnan Province. Biolog microplate method, quantitative real-time PCR, and high-throughput sequencing were used to analyze the microbial community stability, microbial metabolic activity and function diversity, and the abundance of nitrogen-related functional genes. 【Result】Results showed that RSD significantly reduced the stability of bacterial community composition and abundance as compared to CK, with the effects of SB+BD treatment being stronger than that of SB and BD treatment, whereas it had no significant effect on the stability of fungal community composition and abundance. Also, RSD significantly enhanced the stability of interactions between bacterial and fungal communities, and SB and BD treatments had greater effects on the improvement of stability of the interaction between microbial communities than SB+BD treatment. Regression analysis indicated that the stability of interaction relationship of soil microbial community was closely related to the stability of microbial composition and abundance. In addition, correlation analysis showed that soil microbial community stability was highly correlated with its activity, carbon metabolic function and denitrification capacity. 【Conclusion】Collectively, reductive soil disinfestation can improve the stability of interactions between microbial taxa by reducing the stability of microbial community composition and abundance, thereby promoting the restoration of soil microbial activity and improvement of community ecological function.
Zhaojun, Zhangjingqing, Linyulan, Wangbaoying, Huangxinqi, Zhangjinbo, Caizucong. Correlation Between the Stability and Function of Soil Microbial Community Driven by Reductive Soil Disinfestation[J]. Acta Pedologica Sinica, DOI:10.11766/trxb202205150254,[In Press]