【Objective】Reductive soil disinfestations (RSD) is an effective agriculture practice to conquer continuous cropping obstacles by the elimination of soil-borne pathogens, degradation of allelochemicals, improvement of soil microbial community structure, and restoration of soil microbial functions. However, the effects of RSD practice on the stability of the mono-cropped soil microbial community are still unknown. Moreover, the relationship between soil microbial community stability and functions also needs to be studied. Therefore, this study was oriented to explore the relationship between the stability and function of soil microbial communities driven by reductive soil disinfestation.【Method】A field experiment, designed to have four treatments, i.e. CK (control without soil treatment); SB (RSD incorporated with 15 t·hm^-2 organic substrate with C/N ratio of 122); BD (RSD incorporated with 15 t·hm^-2 organic substrate with C/N ratio of 19); and SB+BD (RSD incorporated with 15 t·hm^-2 organic substrates containing both high and low C/N ratios with equal mass), was carried out in a lisianthus mono-cropped soils in Shiping County, Yunnan Province. Biolog microplate method, quantitative real-time PCR, and high-throughput sequencing were used to analyze the microbial community stability, microbial metabolic activity and function diversity, and the abundance of nitrogen-related functional genes.【Result】Results showed that RSD significantly reduced the stability of bacterial community composition and abundance as compared to CK, with the effects of SB+BD treatment being stronger than that of SB and BD treatment, whereas it had no significant effect on the stability of fungal community composition and abundance. Also, RSD significantly enhanced the stability of interactions between bacterial and fungal communities, and SB and BD treatments had greater effects on the improvement of stability of the interaction between microbial communities than SB+BD treatment. Regression analysis indicated that the stability of interaction relationship of soil microbial community was closely related to the stability of microbial composition and abundance. In addition, correlation analysis showed that soil microbial community stability was highly correlated with its activity, carbon metabolic function and denitrification capacity.【Conclusion】Collectively, reductive soil disinfestation can improve the stability of interactions between microbial taxa by reducing the stability of microbial community composition and abundance, thereby promoting the restoration of soil microbial activity and improvement of community ecological function.